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Mouse IL6 Receptor protein (active)

Mouse IL6 Receptor protein (active). GTX00297-pro
Mouse IL6 Receptor protein (active). GTX00297-pro
Mouse IL6 Receptor protein (active). GTX00297-pro

Cat No. GTX00297-pro

Application

Functional Assay

Species

Mouse
Package
10 μg ($249)

APPLICATION

Application Note

IL6R (Interleukin-6 receptor subunit alpha) is part of the receptor for interleukin 6. The interaction of IL6R and IL6 may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Thus, a binding ELISA assay was conducted to detect the association of IL6R with IL6. Briefly, IL6R were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL6-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL6R pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL6R with IL6 was in a dose dependent manner.

Observed MW

18 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from PBS (pH 7.4) with 0.01% SKL, 1 mM DTT, 5% Trehalose, Proclin300. Reconstitute with 10 mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ala52~Ala204 (NP_034689.2)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Mouse IL6 Receptor protein (active). GTX00297-pro

GTX00297-pro Functional Assay Image

Functional ELISA analysis of GTX00297-pro Mouse IL6 Receptor protein (active) which can bind immobilized IL6 protein.

Mouse IL6 Receptor protein (active). GTX00297-pro

GTX00297-pro Image

WB analysis of GTX00297-pro Mouse IL6 Receptor protein (active).

Mouse IL6 Receptor protein (active). GTX00297-pro

GTX00297-pro Image

SDS-PAGE analysis of GTX00297-pro Mouse IL6 Receptor protein (active).

REFERENCE

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REVIEW

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