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Mouse IL6 protein, His tag (active)

Mouse IL6 protein, His tag (active). GTX00286-pro
Mouse IL6 protein, His tag (active). GTX00286-pro
Mouse IL6 protein, His tag (active). GTX00286-pro

Cat. No. GTX00286-pro

Application

 Functional Assay

Species

 Mouse
Package
10 μg ($289)
See all IL6 protein products

APPLICATION

Application Note

Interleukin-6 (IL-6), a pro-inflammatory cytokine and an anti-inflammatory myokine, plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It has been reported that IL6 induces C-reactive protein (CRP) expression through the STAT3 pathway in HepG2 cells. To test this effect, HepG2 cells were seeded overnight at a density of 1×10⁵ cells/ml, and treated with or without various concentrations of IL6 for 24h and CRP levels in the cell supernatant were determined by ELISA. CRP levels in the cell supernatant of HepG2 cells increased significantly after stimulated with IL6.

Observed MW

26-33 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Phe25~Thr211 (NP_001300983.1)

Expression System

HEK293 cells

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 6 , Il-6

Background

This gene encodes a member of the interleukin family of cytokines that have important functions in immune response, hematopoiesis, inflammation and the acute phase response. The ectopic overexpression of the encoded protein in mice results in excessive plasma cells in circulation, leading to death. Mice lacking the encoded protein exhibit abnormalities in hepatic acute phase response, some immune mechanisms, bone resorption in response to estrogen, liver regeneration and wound healing. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Mouse IL6 protein, His tag (active). GTX00286-pro

GTX00286-pro Functional Assay Image

ELISA detection of secreted CRP protein activation from HepG2 cells induced by GTX00286-pro Mouse IL6 protein (active) for 24hrs.
Recombinant protein concentrations: 10, and 30 ng/ml.

Mouse IL6 protein, His tag (active). GTX00286-pro

GTX00286-pro Image

SDS-PAGE analysis of GTX00286-pro Mouse IL6 protein (active).

Mouse IL6 protein, His tag (active). GTX00286-pro

GTX00286-pro Image

WB analysis of GTX00286-pro Mouse IL6 protein (active).

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 289