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Mouse IgG antibody (DyLight594)

Mouse IgG antibody (DyLight594). GTX213111-05
Mouse IgG antibody (DyLight594). GTX213111-05
Mouse IgG antibody (DyLight594). GTX213111-05

Cat No. GTX213111-05

Host Goat
Clonality Polyclonal
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse
Package
500 μl ($149)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

100mM phosphate buffer (pH9.0)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Target Isotype

IgG

Purification

Purified by antigen-affinity chromatography.

Conjugation

DyLight594

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Goat Anti Mouse IgG
DATA IMAGES
Mouse IgG antibody (DyLight594). GTX213111-05

GTX213111-05 IHC-P Image

Double-labeled immunofluorescence photomicrographs of paraffin-embedded sections of mouse colon.
Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. The signal was developed using goat anti-mouse IgG antibody (Dylight594) (GTX213111-05).
Green: E-Cadherin antibody (GTX100443) diluted at 1:500. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Mouse IgG antibody (DyLight594). GTX213111-05

GTX213111-05 ICC/IF Image

alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Red: alpha Tubulin stained by alpha Tubulin antibody (GTX628802) diluted at 1:500.
The signal was developed using Mouse IgG antibody (DyLight594) (GTX213111-05) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Mouse IgG antibody (DyLight594). GTX213111-05

GTX213111-05 IHC-Fr Image

Double-labeled immunofluorescence photomicrographs of frozen sections of mouse brain.
Green: Vimentin antibody (GTX100619) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Red: ME1 antibody [GT15611] (GTX632190) diluted at 1:200. The signal was developed using goat anti-mouse IgG antibody (Dylight594) (GTX213111-05).
Blue: Nuclear staining with Hoechst 33342.

REFERENCE
REVIEW
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SDS
PBS.pdf
Package List Price ($)
$ 149