GeneTex
United States (US)

Mouse IgG antibody (DyLight594)

GTX213111-05_42772_20170426_IHC-P_M_18121410_241.jpg
GTX213111-05_20141103_IFA_18121410_817.jpg
GTX213111-05_20150814_IHC-Fr_M_18121410_753.jpg

Cat No. GTX213111-05

Host Goat
Clonality Polyclonal
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:100-1:2000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

100mM phosphate buffer (pH9.0)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

2mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Target Isotype

IgG

Purification

Purified by antigen-affinity chromatography.

Conjugation

DyLight594

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Goat Anti Mouse IgG
DATA IMAGES
GTX213111-05_42772_20170426_IHC-P_M_18121410_241.jpg

GTX213111-05 IHC-P Image

Double-labeled immunofluorescence photomicrographs of paraffin-embedded sections of mouse colon.
Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. The signal was developed using goat anti-mouse IgG antibody (Dylight594) (GTX213111-05).
Green: E-Cadherin antibody (GTX100443) diluted at 1:500. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX213111-05_20141103_IFA_18121410_817.jpg

GTX213111-05 ICC/IF Image

Immunofluorescent analysis of HeLa cells using alpha Tubulin antibody [GT114] (GTX628802) detected by anti-mouse IgG antibody (Dylight594) (GTX213111-05).
GTX628802 diluted at 1:500 and incubated overnight at 4℃.
GTX213111-05 diluted at 1:2000 and incubated 1 hour at room temperature.

GTX213111-05_20150814_IHC-Fr_M_18121410_753.jpg

GTX213111-05 IHC-Fr Image

Double-labeled immunofluorescence photomicrographs of frozen sections of mouse brain.
Green: Vimentin antibody (GTX100619) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Red: ME1 antibody [GT15611] (GTX632190) diluted at 1:200. The signal was developed using goat anti-mouse IgG antibody (Dylight594) (GTX213111-05).
Blue: Nuclear staining with Hoechst 33342.

REFERENCE
SDS
PBS.pdf
Package List Price ($)
$ 149