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Mouse IgG antibody (HRP)

Mouse IgG antibody (HRP). GTX213111-01
Mouse IgG antibody (HRP). GTX213111-01
Mouse IgG antibody (HRP). GTX213111-01

Cat No. GTX213111-01

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA, IHC
Reactivity Human, Mouse
Package
1 ml ($149)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1:100-1:1000
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

0.05M Tris, 0.15M NaCl (pH7.4), 1%BSA. 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Highly purified whole mouse IgG.

Target Isotype

IgG

Purification

Purified by antigen-affinity chromatography.

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Goat antiMouse IgG HRP, Mouse IgG secondary antibody, antiMouse IgG antibody, anti-Mouse IgG antibody, Goat anti-Mouse IgG HRP, anti Mouse IgG antibody, Goat anti Mouse IgG HRP

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
DATA IMAGES
Mouse IgG antibody (HRP). GTX213111-01

GTX213111-01 IHC-P Image

E2F1 antibody [16G7] detects E2F1 protein at nucleus in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
E2F1 antibody [16G7] (GTX70163) diluted at 1:500.
The signal was developed by Mouse IgG antibody (HRP) (GTX213111-01).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Mouse IgG antibody (HRP). GTX213111-01

GTX213111-01 WB Image

Various whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Mouse IgG antibody (HRP). GTX213111-01

GTX213111-01 WB Image

Various amounts of denatured mouse IgG protein were separated by 12% SDS-PAGE, and the membrane was blotted with HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) diluted at 1:5000.

REFERENCE
REVIEW
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Application Reference
SDS
Proclin.pdf
Package List Price ($)
$ 149