GeneTex
United States (US)

Mouse IgG antibody (HRP)

GTX213112-01_41628_20150529_ELISA_18121410_898.jpg

Cat No. GTX213112-01

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ELISA
Reactivity Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:10000
ELISA Assay dependent
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

0.05M Tris, 0.15M NaCl (pH7.4), 1%BSA. 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.59 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Highly purified whole mouse IgG

Target Isotype

IgG

Purification

Affinity purified with antigen
Affinity Purified IgG

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mouse IgG secondary antibody, antiMouse IgG antibody, anti-Mouse IgG antibody, Rabbit antiMouse IgG HRP, Rabbit anti-Mouse IgG HRP, anti Mouse IgG antibody, Rabbit anti Mouse IgG HRP

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
DATA IMAGES
GTX213112-01_41628_20150529_ELISA_18121410_898.jpg

GTX213112-01 ELISA Image

An ELISA plate is coated with 50 μL of a 6x-His recombinant protein conjugated BSA at concentration ranged from 0.003 μg/mL to 3 μg/mL. The coated protein is detected with anti-His antibody (GTX628921) at 0.5 ng/mL. Bound antibodies were further detected by using HRP-conjugated goat anti-mouse IgG (GTX213112-01).

REFERENCE
SDS
Proclin.pdf
Package List Price ($)
$ 149