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Mouse IgG isotype control

Cat No. GTX35009

Host Mouse
Clonality Polyclonal
Isotype IgG
Application ICC/IF, FACS, IP, ELISA
Package
100 μg ($79)
APPLICATION

Application Note

Use at a concentration/dilution equivalent to that of your experimental primary antibody.
PROPERTIES

Form

Liquid

Buffer

100 mM borate buffered saline, pH 8.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Isotype control for Mouse IgG, Polyclonal Mouse IgG isotype control, Mouse IgG negative control, Mouse IgG isotype control, Negative control for Mouse IgG, Mouse isotype control for IgG, Isotype control to Mouse IgG, Mouse negative control for IgG

Background

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).
REFERENCE
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Application Reference
Package List Price ($)
$ 79