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Mouse IgG1 isotype control [15H6] (PE)

Cat No. GTX35017

Host

Mouse

Clonality

Monoclonal

Clone Name

15H6

Isotype

IgG1

Application

FACS, ELISA
Package
50 test ($99)

APPLICATION

Application Note

Use at a concentration/dilution equivalent to that of your experimental primary antibody.

Specificity/Sensitivity

T-2 mycotoxin

PROPERTIES

Form

Liquid

Buffer

PBS + 0.1% NaN3 and a stabilizing agent

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. Protect from light.

Immunogen

T-2 mycotoxin

Conjugation

Phycoerythrin (PE)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Mouse IgG1 negative control , Mouse IgG1 isotype control , Mouse isotype control for IgG1 kappa , Negative control for Mouse IgG1 kappa , Mouse IgG1 kappa negative control , Mo , monoclonal Mouse IgG1 isotype control , Isotype control for Mouse IgG1 kappa

Background

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 99