GeneTex
United States (US)

Mouse IgG1 isotype control [15H6]

Cat No. GTX35014

Host Mouse
Clonality Monoclonal
Isotype IgG1
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, ELISA, Blocking, in vitro, in vivo
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
ELISA Assay dependent
Blocking Assay dependent
in vitro Assay dependent
in vivo Assay dependent
Not tested in other applications.

Specificity/Sensitivity

T-2 mycotoxin
PROPERTIES

Form

Liquid

Buffer

Supplied in borate buffered saline, pH 8.2 without any preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1mg/ml

Immunogen

T-2 mycotoxin

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mouse IgG1 negative control, Mouse IgG1 isotype control, Mouse isotype control for IgG1 kappa, Negative control for Mouse IgG1 kappa, Mouse IgG1 kappa negative control, Mo, monoclonal Mouse IgG1 isotype control, Isotype control for Mouse IgG1 kappa

Background

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).
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Reference
Package List Price ($)
$ 79