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Mouse IgG2a isotype control [HOPC-1]

Cat No. GTX35019

Host

Mouse

Clonality

Monoclonal

Clone Name

HOPC-1

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, in vitro, in vivo
Package
100 μg ($79)

APPLICATION

Application Note

Use at a concentration/dilution equivalent to that of your experimental primary antibody.

Specificity/Sensitivity

Unknown

PROPERTIES

Form

Liquid

Buffer

100 mM borate buffered saline, pH 8.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Negative control for Mouse IgG2a kappa, Isotype control to Mouse IgG2a kappa, monoclonal Mouse IgG2a isotype control, Mouse IgG2a negative control, Mouse isotype control for IgG2a kappa, Mouse IgG2a kappa isot, Mouse negative control for IgG2a kappa

Background

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).

REFERENCE

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Package List Price ($)
$ 79