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Mouse Insulin protein (active)

Mouse Insulin protein (active). GTX00291-pro
Mouse Insulin protein (active). GTX00291-pro
Mouse Insulin protein (active). GTX00291-pro
Mouse Insulin protein (active). GTX00291-pro

Cat No. GTX00291-pro

Application Functional Assay
Species Mouse
Package
10 μg ($249)
APPLICATION

Application Note

INS (Insulin) is a peptide hormone produced by beta cells of the pancreatic islets, which decreases blood glucose concentration and increases cell permeability to monosaccharides, amino acids and fatty acids. It has been reported that insulin triggers phosphorylation of a number of substrates by binding to its receptors, which was important for cell proliferation, cell cycle progression, cell division and differentiation. To detect the effect of Insulin on cell proliferation,  MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM  prior to the addition of various concentrations of INS. After incubated for 48h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. The dose-effect curve showed INS significantly promoted cell proliferation of MCF-7 cells. The ED₅₀ for this effect is typically 11. 71-57. 11 ng/ml.

Observed MW

10 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Phe25~Ser54(B)+GGGGS+Gly90~Asn110(A) (NP_001172012.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

insulin II,AA986540,Ins-2,InsII,Mody,Mody4

Background

This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Database

Research Area

DATA IMAGES
Mouse Insulin protein (active). GTX00291-pro

GTX00291-pro Functional Assay Image

The dose effect curve of GTX00291-pro Mouse Insulin protein (active) on MCF-7 cells. After incubation of Mouse Insulin protein (active) for 48 hrs, cell proliferation was measured by Cell Counting Kit-8 (CCK-8).

Mouse Insulin protein (active). GTX00291-pro

GTX00291-pro Functional Assay Image

Cell proliferation effect of GTX00291-pro Mouse Insulin protein (active).
(A) Unstimulated MCF-7 cells cultured in DMEM for 72hrs.
(B) MCF-7 cells cultured in DMEM, stimulated with 100 ng/ml INS for 72hrs.

Mouse Insulin protein (active). GTX00291-pro

GTX00291-pro Image

SDS-PAGE analysis of GTX00291-pro Mouse Insulin protein (active).

Mouse Insulin protein (active). GTX00291-pro

GTX00291-pro Image

WB analysis of GTX00291-pro Mouse Insulin protein (active).

REFERENCE
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Package List Price ($)
$ 249