APPLICATION
Application Note
1. Measured in antiviral assay using L929 cells infected with VSV (vesicular stomatitis virus). The ED₅₀ for this effect is typically 0.2-1 ng/ml.
2. Measured by its binding ability in a functional ELISA. Immobilized Mouse Interferon gamma protein (active) (GTX01303-pro) at 10 μg/ml (100 μl/well) can bind Mouse Interferon gamma Receptor 1 protein (active) (GTX01441-pro). The EC₅₀ of Mouse Interferon gamma Receptor 1 protein (active) (GTX01441-pro) is 58.2-135.9 ng/ml.
Calculated MW
Observed MW
21 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Batch dependent (please contact us for details)
Storage
Store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
C-terminal poly His tagged; Met1-Cys155 of Mouse Interferon gamma protein (P01580)
Expression System
HEK293 cells
Purity
> 75% by SDS-PAGE.
Endotoxin
< 1.0 EU/μg (determined by LAL method)
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
interferon gamma , IFN-g , Ifg
Cellular Localization
Secreted
Background
This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mice deficient in this gene have increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]
Database
Research Area
DATA IMAGES
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GTX01303-pro Functional Assay Image
The protein activity of GTX01303-pro Mouse Interferon gamma protein was measured in antiviral assays using L929 cells infected with VSV (vesicular stomatitis virus).
ED₅₀ : 0.2-1 ng/ml
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GTX01303-pro Functional Assay Image
The protein binding activity was measured in a functional ELISA analysis of GTX01441-pro Mouse Interferon gamma Receptor 1 protein which can bind immobilized GTX01303-pro Mouse Interferon gamma protein.
Immobilized GTX01303-pro : 10 μg/ml (100 μl/well)
GTX01441-pro : 58.2-135.9 ng/ml
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GTX01303-pro Image
SDS-PAGE of 5 μg GTX01303-pro Mouse Interferon gamma protein, His tag (active).
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REFERENCE
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REVIEW
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