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Mouse Interferon gamma protein, His tag (active)

Mouse Interferon gamma protein, His tag (active). GTX01303-pro
Mouse Interferon gamma protein, His tag (active). GTX01303-pro
Mouse Interferon gamma protein, His tag (active). GTX01303-pro

Cat No. GTX01303-pro

Application

Functional Assay

Species

Mouse
Package
100 μg ($279),
20 μg ($199)

APPLICATION

Application Note

1. Measured in antiviral assay using L929 cells infected with VSV (vesicular stomatitis virus). The ED₅₀ for this effect is typically 0.2-1 ng/ml.
2. Measured by its binding ability in a functional ELISA. Immobilized Mouse Interferon gamma protein (active) (GTX01303-pro) at 10 μg/ml (100 μl/well) can bind Mouse Interferon gamma Receptor 1 protein (active) (GTX01441-pro). The EC₅₀ of Mouse Interferon gamma Receptor 1 protein (active) (GTX01441-pro) is 58.2-135.9 ng/ml.

Calculated MW

18 kDa. ( Note )

Observed MW

21 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Batch dependent (please contact us for details)

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

C-terminal poly His tagged; Met1-Cys155 of Mouse Interferon gamma protein (P01580)

Expression System

HEK293 cells

Purity

> 75% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interferon gamma , IFN-g , Ifg

Cellular Localization

Secreted

Background

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mice deficient in this gene have increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Mouse Interferon gamma protein, His tag (active). GTX01303-pro

GTX01303-pro Functional Assay Image

The protein activity of GTX01303-pro Mouse Interferon gamma protein was measured in antiviral assays using L929 cells infected with VSV (vesicular stomatitis virus).
ED₅₀ : 0.2-1 ng/ml

Mouse Interferon gamma protein, His tag (active). GTX01303-pro

GTX01303-pro Functional Assay Image

The protein binding activity was measured in a functional ELISA analysis of GTX01441-pro Mouse Interferon gamma Receptor 1 protein which can bind immobilized GTX01303-pro Mouse Interferon gamma protein.
Immobilized GTX01303-pro : 10 μg/ml (100 μl/well)
GTX01441-pro : 58.2-135.9 ng/ml

Mouse Interferon gamma protein, His tag (active). GTX01303-pro

GTX01303-pro Image

SDS-PAGE of 5 μg GTX01303-pro Mouse Interferon gamma protein, His tag (active).

REFERENCE

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REVIEW

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Package List Price ($)
$ 279
$ 199