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Mouse Interferon gamma protein, His tag (active)

Mouse Interferon gamma protein, His tag (active). GTX00283-pro
Mouse Interferon gamma protein, His tag (active). GTX00283-pro
Mouse Interferon gamma protein, His tag (active). GTX00283-pro
Mouse Interferon gamma protein, His tag (active). GTX00283-pro

Cat. No. GTX00283-pro

Application

 Functional Assay

Species

 Mouse
Package
10 μg ($229)
See all Interferon gamma protein products

APPLICATION

Application Note

IFN-γ is an important activator of macrophages, it promotes production of inducible Nitric Oxide Synthase (iNOS) in macrophages. After stimulated with IFN-γ, morphological changes will occur in murine macrophage cell line (Raw 246. 7 cells), and inducible nitric-oxide synthase (iNOS) in the cells will increase. Raw 246. 7 cells were incubated in DMEM with IFN-γ (2 ng/ml) for 24h, then cells  were  observed  by  inverted  microscope.

Observed MW

17 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; His23~Cys155 (NP_032363.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interferon gamma , IFN-g , Ifg

Background

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mice deficient in this gene have increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Mouse Interferon gamma protein, His tag (active). GTX00283-pro

GTX00283-pro Functional Assay Image

Activation effect of GTX00283-pro Mouse Interferon gamma protein (active).
(A) Unstimulated Raw 264.7 cells cultured in DMEM.
(B) Raw 264.7 cells cultured in DMEM, stimulated with IFN gamma.

Mouse Interferon gamma protein, His tag (active). GTX00283-pro

GTX00283-pro Functional Assay Image

ELISA detection of iNOS protein expression from RAW 246.7 cells stimulated by GTX00283-pro Mouse Interferon gamma protein (active) at 2 ng/ml for 24hrs.

Mouse Interferon gamma protein, His tag (active). GTX00283-pro

GTX00283-pro Image

SDS-PAGE analysis of GTX00283-pro Mouse Interferon gamma protein (active).

Mouse Interferon gamma protein, His tag (active). GTX00283-pro

GTX00283-pro Image

WB analysis of GTX00283-pro Mouse Interferon gamma protein (active).

REFERENCE

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SDS
Proclin.pdf
Package List Price ($)
$ 229