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Mouse LCAT protein (active)

Mouse LCAT protein (active). GTX00300-pro
Mouse LCAT protein (active). GTX00300-pro
Mouse LCAT protein (active). GTX00300-pro

Cat No. GTX00300-pro

Application Functional Assay
Species Mouse
Package
10 μg ($309)
APPLICATION

Application Note

LCAT (Phosphatidylcholine-sterol acyltransferase) is an enzyme in the extracellular metabolism of plasma lipoproteins, which converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines. It is reported that APOA1 (Apolipoprotein A-I) acts as a cofactor for the LCAT. Thus, a binding ELISA assay was conducted to detect the association of LCAT with APOA1. Briefly, recombinant rat LCAT were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to APOA1-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-LCAT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of LCAT with APOA1 was in a dose dependent manner.

Observed MW

22 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from PBS (pH 7.4) with 0.01% SKL, 1 mM DTT, 5% Trehalose, Proclin300. Reconstitute with 10 mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Glu41~His210 (NP_032516.2)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

lecithin cholesterol acyltransferase , AI046659 , D8Wsu61e

Database

Research Area

DATA IMAGES
Mouse LCAT protein (active). GTX00300-pro

GTX00300-pro Functional Assay Image

Functional ELISA analysis of GTX00300-pro Mouse LCAT protein (active) which can bind immobilized APOA1 protein.

Mouse LCAT protein (active). GTX00300-pro

GTX00300-pro Image

SDS-PAGE analysis of GTX00300-pro Mouse LCAT protein (active).

Mouse LCAT protein (active). GTX00300-pro

GTX00300-pro Image

WB analysis of GTX00300-pro Mouse LCAT protein (active).

REFERENCE
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