GeneTex
  • Country / Location Selection

United States (US)

Mouse LCAT protein (active)

Mouse LCAT protein (active). GTX00300-pro
Mouse LCAT protein (active). GTX00300-pro
Mouse LCAT protein (active). GTX00300-pro

Cat No. GTX00300-pro

Application Functional Assay
Species Mouse
Package
10 μg ($309)
APPLICATION

Application Note

LCAT (Phosphatidylcholine-sterol acyltransferase) is an enzyme in the extracellular metabolism of plasma lipoproteins, which converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines. It is reported that APOA1 (Apolipoprotein A-I) acts as a cofactor for the LCAT. Thus, a binding ELISA assay was conducted to detect the association of LCAT with APOA1. Briefly, recombinant rat LCAT were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to APOA1-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-LCAT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of LCAT with APOA1 was in a dose dependent manner.

Observed MW

22 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from PBS (pH 7.4) with 0.01% SKL, 1 mM DTT, 5% Trehalose, Proclin300. Reconstitute with 10 mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Glu41~His210 (NP_032516.2)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

lecithin cholesterol acyltransferase,AI046659,D8Wsu61e

Database

Research Area

DATA IMAGES
Mouse LCAT protein (active). GTX00300-pro

GTX00300-pro Functional Assay Image

Functional ELISA analysis of GTX00300-pro Mouse LCAT protein (active) which can bind immobilized APOA1 protein.

Mouse LCAT protein (active). GTX00300-pro

GTX00300-pro Image

SDS-PAGE analysis of GTX00300-pro Mouse LCAT protein (active).

Mouse LCAT protein (active). GTX00300-pro

GTX00300-pro Image

WB analysis of GTX00300-pro Mouse LCAT protein (active).

REFERENCE
There are currently no references for Mouse LCAT protein (active) (GTX00300-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Mouse LCAT protein (active) (GTX00300-pro). Be the first to share your experience with this product.