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Mouse M-CSF Receptor protein, human IgG1 Fc and His tag (active)

Mouse M-CSF Receptor protein, human IgG1 Fc and His tag (active). GTX01381-pro
Mouse M-CSF Receptor protein, human IgG1 Fc and His tag (active). GTX01381-pro

Cat No. GTX01381-pro

Application

Functional Assay

Species

Mouse
Package
100 μg ($329),
50 μg ($249)

APPLICATION

Application Note

1. Immobilized Mouse M-CSF protein (active) (GTX01307-pro) at 10 μg/ml (100 μl/well) can bind Mouse M-CSF Receptor protein (active) (GTX01381-pro). The EC₅₀ of mouse CSF1R-human IgG1 Fc protein is 0.04-0.1 μg/ml.
2. Measured by its ability to inhibit mouse CSF-induced proliferation of M-NFS-60 cells. The ED₅₀ for this effect is typically 0.01-0.04 μg/ml in the presence of 3 ng/ml recombinant human M-CSF protein.
3. Immobilized Mouse M-CSF protein (active) (GTX01306-pro) at 10 μg/ml (100 μl/well) can bind Mouse M-CSF Receptor protein (active) (GTX01381-pro). The EC₅₀ of Mouse M-CSF Receptor protein (active) (GTX01381-pro) is 0.04-0.1 μg/ml.

Calculated MW

109 kDa. ( Note )

Observed MW

110 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Batch dependent (please contact us for details)

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

C-terminal human IgG1-Fc and poly His tagged; Met1-Ser511 of Mouse M-CSF Receptor protein (NP_001032948.2)

Expression System

HEK293 cells

Purity

> 95% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

colony stimulating factor 1 receptor , AI323359 , CD115 , CSF-1R , Csfmr , Fim-2 , Fim2 , Fms , M-CSF-R , M-CSFR

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Mouse M-CSF Receptor protein, human IgG1 Fc and His tag (active). GTX01381-pro

GTX01381-pro Functional Assay Image

The protein activity of GTX01381-pro Mouse M-CSF Receptor protein was measured by its ability to inhibit mouse CSF-induced proliferation of M-NFS-60 cells.
ED₅₀ : 0.01-0.04 μg/ml in the presence of 3 ng/ml recombinant mouse M-CSF protein

Mouse M-CSF Receptor protein, human IgG1 Fc and His tag (active). GTX01381-pro

GTX01381-pro Image

SDS-PAGE of 5 μg GTX01381-pro Mouse M-CSF Receptor protein, human IgG1 Fc and His tag (active).

REFERENCE

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REVIEW

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