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Mouse MIF protein (active)

Mouse MIF protein (active). GTX00313-pro
Mouse MIF protein (active). GTX00313-pro
Mouse MIF protein (active). GTX00313-pro

Cat No. GTX00313-pro

Application

Functional Assay

Species

Mouse
Package
10 μg ($319)

APPLICATION

Application Note

Macrophage migration inhibitory factor (MIF), also known as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase is a protein classified as an inflammatory cytokine. MIF is an important regulator of innate immunity. It involved in cell-mediated immunity, immunoregulation, and inflammation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate a role in integrin signaling pathways. Besides, Major Histocompatibility Complex Class II Invariant Chain (MHCDG) has been identified as an interactor of MIF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse MIF and recombinant mouse MHCDG. Briefly, MIF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to MHCDG-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-MIF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of MIF and MHCDG was in a dose dependent manner.

Observed MW

14 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Phe4~Ser112 (NP_034928.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

macrophage migration inhibitory factor (glycosylation-inhibiting factor) , DER6 , GIF , Glif

Database

Research Area

DATA IMAGES

Mouse MIF protein (active). GTX00313-pro

GTX00313-pro Functional Assay Image

Functional ELISA analysis of GTX00313-pro Mouse MIF protein (active) which can bind immobilized MHCDG protein.

Mouse MIF protein (active). GTX00313-pro

GTX00313-pro Image

SDS-PAGE analysis of GTX00313-pro Mouse MIF protein (active).

Mouse MIF protein (active). GTX00313-pro

GTX00313-pro Image

WB analysis of GTX00313-pro Mouse MIF protein (active).

REFERENCE

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REVIEW

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