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Mouse PDGFA protein, His tag (active)

Mouse PDGFA protein, His tag (active). GTX00292-pro
Mouse PDGFA protein, His tag (active). GTX00292-pro
Mouse PDGFA protein, His tag (active). GTX00292-pro

Cat. No. GTX00292-pro

Application

 Functional Assay

Species

 Mouse
Package
10 μg ($289)
See all PDGFA protein products

APPLICATION

Application Note

PDGFA (Platelet-derived growth factor subunit A) is a Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. PDGFA has been described as a chemoattractant for monocytes and proven to be able to induce chemotactic migration of THP-1 cells. Therefore, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of PDGFA on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100 μl cell suspension, 1x10⁶cells/ml in RPMI-1640 with 0. 5% FBS) and PDGFA (1. 5 ng/ml, 7. 5 ng/ml and 15 ng/ml diluted separately in serum free RPMI-1640 ) was added in lower chamber with a polycarbonate filter (8μm pore size) used to separate the two compartments. After incubation at 37ºC with 5% CO₂ for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×40) and the number of migrated cells were counted at high magnification (×400) randomly. PDGFA is able to induce migration of THP-1 cells, and the optimum chemotaxis of PDGFA occurs at 1. 5 ng/ml.

Observed MW

17 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ala94~Asp194 (NP_032834.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

platelet derived growth factor, alpha , PDGF-1

Database

Research Area

DATA IMAGES

Mouse PDGFA protein, His tag (active). GTX00292-pro

GTX00292-pro Functional Assay Image

The chemotactic effect of GTX00292-pro Mouse PDGFA protein (active).
Upper: (A) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 without PDGFA was added in lower chamber, then cells in lower chamber were observed at low magnification (×40) after incubation for 3hrs.
(B) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 with 1.5 ng/ml PDGFA was added in lower chamber, then cells in lower chamber were observed at low magnification (×40) after incubation for 3hrs.
Lower: The number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter).

Mouse PDGFA protein, His tag (active). GTX00292-pro

GTX00292-pro Image

WB analysis of GTX00292-pro Mouse PDGFA protein (active).

Mouse PDGFA protein, His tag (active). GTX00292-pro

GTX00292-pro Image

SDS-PAGE analysis of GTX00292-pro Mouse PDGFA protein (active).

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 289