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Mouse PF4 protein, His tag (active)

Mouse PF4 protein, His tag (active). GTX00334-pro
Mouse PF4 protein, His tag (active). GTX00334-pro
Mouse PF4 protein, His tag (active). GTX00334-pro
Mouse PF4 protein, His tag (active). GTX00334-pro

Cat. No. GTX00334-pro

Reference 1

Application

 Functional Assay

Species

 Mouse
Package
10 μg ($299)
See all PF4 protein products

APPLICATION

Application Note

Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). This chemokine is released from alpha-granules of activated platelets during platelet aggregation, and promotes blood coagulation by moderating the effects of heparin-like molecules. Due to these roles, it is predicted to play a role in wound repair and inflammation. To measure its ability to inhibit the FGF basic-dependent proliferation of HUVEC human umbilical vein endothelial cells, HUVEC cells were seeded into 96-well plates at a density of 3000 cells/well with 2% serum standard DMEM including 1μg/ml recombinant human FGF1 and various concentrations of recombinant human PF4. After incubated for 48h, cells were observed by inverted microscope. Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-2 hours at 37ºC. Proliferation of HUVEC cells after incubation with PF4 for 48h observed by inverted microscope.

Observed MW

13 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Val30~Ser105 (NP_064316.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

platelet factor 4 , Cxcl4 , Scyb4

Database

Research Area

DATA IMAGES

Mouse PF4 protein, His tag (active). GTX00334-pro

GTX00334-pro Functional Assay Image

The inhibition effect of GTX00334-pro Mouse PF4 protein (active) on FGF basic-dependent proliferation.
(A) Unstimulated HUVEC cells cultured in DMEM with 1 μg/ml FGF1 for 48hrs.
(B) HUVEC cells cultured in DMEM with 1 μg/ml FGF1, stimulated with 5 μg/ml PF4 for 48hrs.

Mouse PF4 protein, His tag (active). GTX00334-pro

GTX00334-pro Functional Assay Image

The inhibitory effect of GTX00334-pro Mouse PF4 protein (active) on the FGF basic-dependent proliferation of HUVEC. After incubation of 1 μg/ml of recombinant human FGF1 protein and various concentraions of Mouse PF4 protein (active) for 48 hrs, cell viability was measured by Cell Counting Kit-8 (CCK-8).

Mouse PF4 protein, His tag (active). GTX00334-pro

GTX00334-pro Image

WB analysis of GTX00334-pro Mouse PF4 protein (active).

Mouse PF4 protein, His tag (active). GTX00334-pro

GTX00334-pro Image

SDS-PAGE analysis of GTX00334-pro Mouse PF4 protein (active).

REFERENCE

Application Reference

Park C et al. Nat Aging 2023; 3 (9) : 1067-1078 Platelet factors are induced by longevity factor klotho and enhance cognition in young and aging mice.
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SDS
Proclin.pdf
Package List Price ($)
$ 299