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Mouse PTHrP protein, His tag

Mouse PTHrP protein, His tag. GTX00047-pro
Mouse PTHrP protein, His tag. GTX00047-pro
Mouse PTHrP protein, His tag. GTX00047-pro

Cat. No. GTX00047-pro

Application

 Functional Assay

Species

 Mouse
Package
10 μg ($299)
See all PTHrP protein products

APPLICATION

Application Note

PTHrP (Parathyroid Hormone Related Protein ) is a neuroendocrine peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport. Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. A binding ELISA assay was conducted to detect the association of PTHrP with PTHR1. Briefly, PTHrP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl PTHrP were then transferred to PTHR1-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-PTHrP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of PTHrP and PTHR1 was in a dose dependent manner.

Observed MW

18/19 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ala37~His175

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

parathyroid hormone-like peptide , PLP , PTH-like , Pthrp

Background

This gene encodes a member of the parathyroid family of hormones that possesses distinct paracrine and intracrine signaling roles such as regulation of circulating calcium, transplacental calcium transport, osteoclast inhibition, renal bicarbonate excretion and regulation of apoptosis. The encoded protein undergoes proteolytic processing to generate multiple active peptides with distinct signaling functions. The homozygous deletion of this gene leads to death shortly after birth with a chondrodystrophic phenotype characterized by premature chondrocyte differentiation and accelerated bone formation. [provided by RefSeq, Jul 2015]

Database

Research Area

DATA IMAGES

Mouse PTHrP protein, His tag. GTX00047-pro

GTX00047-pro Functional Assay Image

Functional ELISA analysis of GTX00047-pro Mouse PTHrP protein which can bind immobilized PTHR1 protein.

Mouse PTHrP protein, His tag. GTX00047-pro

GTX00047-pro Image

WB analysis of GTX00047-pro Mouse PTHrP protein.

Mouse PTHrP protein, His tag. GTX00047-pro

GTX00047-pro Image

SDS-PAGE analysis of GTX00047-pro Mouse PTHrP protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 299