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Mouse Perforin protein (active)

Mouse Perforin protein (active). GTX00328-pro
Mouse Perforin protein (active). GTX00328-pro
Mouse Perforin protein (active). GTX00328-pro

Cat No. GTX00328-pro

Application Functional Assay
Species Mouse
Package
10 μg ($309)
APPLICATION

Application Note

Perforin 1 (PRF1) is a pore forming cytolytic protein found in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, perforin binds to the target cell'Splasma membrane, and oligomerises in a Ca2 dependent manner to form pores on the target cell. Perforin is thought to act by creating holes in the plasma membrane which triggers an influx of calcium and initiates membrane repair mechanisms. These repair mechanisms bring perforin and granzymes into early endosomes. Besides, Granzyme A (GZMA) has been identified as an interactor of PRF1, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse PRF1 and recombinant mouse GZMA. Briefly, PRF1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to GZMA-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-PRF1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of PRF1 and GZMA was in a dose dependent manner.

Observed MW

65 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Val40~Lys355 (NP_035203.3)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

perforin 1 (pore forming protein) , Pfn , Pfp , Prf-1

Database

Research Area

DATA IMAGES
Mouse Perforin protein (active). GTX00328-pro

GTX00328-pro Functional Assay Image

Functional ELISA analysis of GTX00328-pro Mouse Perforin protein (active) which can bind immobilized GZMA protein.

Mouse Perforin protein (active). GTX00328-pro

GTX00328-pro Image

SDS-PAGE analysis of GTX00328-pro Mouse Perforin protein (active).

Mouse Perforin protein (active). GTX00328-pro

GTX00328-pro Image

WB analysis of GTX00328-pro Mouse Perforin protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 309