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Mouse Progranulins protein

Mouse Progranulins protein. GTX65642-pro
Mouse Progranulins protein. GTX65642-pro

Cat No. GTX65642-pro

Species

Mouse
Package
10 μg ($299)

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 0.2μm-filtered PBS. Reconstitute with 100 μl ddH₂O.

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Aliquot and avoid freeze-thaw cycles.

Region/Sequence

Signal peptide and mouse progranulin (a.a. 1-589); reflects the native with no additional a.a. .

Expression System

HEK293 cells

Purity

≥ 95% by SDS-PAGE.

Endotoxin

< 0.01 EU/μg

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

granulin , GP88 , PCDGF , PEPI , Pgrn , epithelin

Database

Research Area

DATA IMAGES

Mouse Progranulins protein. GTX65642-pro

GTX65642-pro Image

Deglycosylation of GTX65642-pro Progranulin (mouse) protein.To examine the deglycosylation of mouse Progranulin, 1 μg of mouse progranulin is denatured with 1X glycoprotein denaturing buffer at 100ºC for 10 minutes. After the addition of NP-40 and G7 reaction buffer, two fold dilutions of PNGase F are added and the reaction mix is incubated for 1 hour at 37ºC. Separation of reaction products is visualized by immunoblotting using anti-Progranulin (mouse) antibody.

Mouse Progranulins protein. GTX65642-pro

GTX65642-pro Image

The effects on phospho-ERK1/2 and non-phospho-ERK1/2 by GTX65642-pro Progranulin (mouse) protein in neuronal differentiated mouse P19 cells.Undifferentiated mouse P19 were induced to differentiated in 1μM retinoic acid (RA) in α-minimum essential medium (αMEM) containing 10% heat-treated fetal bovine serum on bacterial grade plates for 3~4 days to allow aggregates to form (generation of embryonic bodies). The aggregates were then plated on tissue culture grade plates in the absence of RA for 3~4days. To examine the induction of signal of phospho-ERK1/2 and ERK1/2 , reactions were carried out at 37ºC over 0, 5, 10, 30, 60, 120mins, respectively by adding the recombinant protein (500ng/ml) to the neuronal differentiated mouse P19 embryonal carcinoma cells, which were maintained with serum starvation for 24hrs. Treatment with GTX65642-pro Progranulin (mouse) protein was performed in lanes 1, 2, 3, 4, 5, and 6 over 0, 5, 10, 30, 60, 120mins, respectively.

REFERENCE

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REVIEW

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SDS
PBS.pdf