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Mouse RANKL protein (active)

Mouse RANKL protein (active). GTX00319-pro
Mouse RANKL protein (active). GTX00319-pro
Mouse RANKL protein (active). GTX00319-pro

Cat No. GTX00319-pro

Application Functional Assay
Species Mouse
Package
10 μg ($269)
APPLICATION

Application Note

Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF), is a protein that in humans is encoded by the TNFSF11 gene. RANKL is a member of the tumor necrosis factor (TNF) cytokine family, it binds to RANK on cells of the myeloid lineage and functions as a key factor for osteoclast differentiation and activation. It may also bind to osteoprotegerin, a protein secreted mainly by cells of the osteoblast lineage which is a potent inhibitor of osteoclast formation by preventing binding of RANKL to RANK. RANKL also has a function in the immune system, where it is expressed by T helper cells and is thought to be involved in dendritic cell maturation. This protein was shown to be a dendritic cell survival factor and is involved in the regulation of T cell-dependent immune response. Besides, Peroxisome Proliferator Activated Receptor Gamma (PPARg) has been identified as an interactor of RANKL, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse RANKL and recombinant mouse PPARg. Briefly, RANKL were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PPARg-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-RANKL pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of RANKLand PPARg was in a dose dependent manner.

Observed MW

49 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Leu92~Trp263 (NP_035743.2)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

tumor necrosis factor (ligand) superfamily, member 11,Ly109l,ODF,OPGL,RANKL,Trance

Database

Research Area

DATA IMAGES
Mouse RANKL protein (active). GTX00319-pro

GTX00319-pro Functional Assay Image

Functional ELISA analysis of GTX00319-pro Mouse RANKL protein (active) which can bind immobilized PPARg protein.

Mouse RANKL protein (active). GTX00319-pro

GTX00319-pro Image

SDS-PAGE analysis of GTX00319-pro Mouse RANKL protein (active).

Mouse RANKL protein (active). GTX00319-pro

GTX00319-pro Image

WB analysis of GTX00319-pro Mouse RANKL protein (active).

REFERENCE
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