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Mouse Reg3B protein (active)

Mouse Reg3B protein (active). GTX00307-pro
Mouse Reg3B protein (active). GTX00307-pro
Mouse Reg3B protein (active). GTX00307-pro
Mouse Reg3B protein (active). GTX00307-pro

Cat No. GTX00307-pro

Application Functional Assay
Species Mouse
Package
10 μg ($309)
APPLICATION

Application Note

Regenerating Islet Derived Protein 3 Beta (REG3b) also known as PAP-I and HIP is bactericidal C-type lectin which acts against several intestinal Gram-positive bacteria and Gram-negative bacteria. The Reg family proteins have been implicated in a range of physiological processes including acting as acute phase reactants, lectins, survival/growth factors for insulin-producing pancreatic beta-cells, neural cells, and epithelial cells of the digestive system. To test the effect of REG3b on cell proliferation of SK-N-SH, SK-N-SH cells were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM  prior to the addition of various concentrations of REG3b. After incubated for 72h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37ºC . Cell proliferation of SK-N-SH cells after incubation with REG3b for 72h observed by inverted microscope.

Observed MW

17 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Glu27~Gly175 (NP_035166.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

regenerating islet-derived 3 beta,HIP,PAP1,Pap,REG-III

Database

DATA IMAGES
Mouse Reg3B protein (active). GTX00307-pro

GTX00307-pro Functional Assay Image

Cell proliferation effect of GTX00307-pro Mouse Reg3B protein (active).
(A) Unstimulated SK-N-SH cells cultured in serum-free DMEM for 72hrs.
(B) SK-N-SH cells cultured in DMEM, stimulated with 10 ng/ml REG3b 72hrs.

Mouse Reg3B protein (active). GTX00307-pro

GTX00307-pro Functional Assay Image

The dose effect curve of GTX00307-pro Mouse Reg3B protein (active) on SK-N-SH cells. After incubation of Mouse Reg3B protein (active) for 72 hrs, cell proliferation was measured by Cell Counting Kit-8 (CCK-8).

Mouse Reg3B protein (active). GTX00307-pro

GTX00307-pro Image

WB analysis of GTX00307-pro Mouse Reg3B protein (active).

Mouse Reg3B protein (active). GTX00307-pro

GTX00307-pro Image

SDS-PAGE analysis of GTX00307-pro Mouse Reg3B protein (active).

REFERENCE
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Package List Price ($)
$ 309