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Mouse S100 beta protein (active)

Mouse S100 beta protein (active). GTX00294-pro
Mouse S100 beta protein (active). GTX00294-pro
Mouse S100 beta protein (active). GTX00294-pro

Cat No. GTX00294-pro

Application Functional Assay
Species Mouse
Package
10 μg ($249)
APPLICATION

Application Note

Protein S100B is a member of the S100 family. S100 proteins are EF-hand calcium-binding proteins and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Experimental results suggest that the receptor for advanced glycation end products (RAGE) plays important roles in mediating S100 protein-induced cellular signaling. Besides, mouse RAGE shares similarities with human RAGE in amino acids sequence with the identity of 78.2% . Thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse S100B and recombinant human RAGE. Briefly, S100B were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-S100B mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of S100B and RAGE was in a dose dependent manner.

Observed MW

10 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

Full length protein, N-terminal His-Tag; Met1~Glu92 (NP_033141.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

S100 protein, beta polypeptide, neural,AI850290,Bpb

Database

Research Area

DATA IMAGES
Mouse S100 beta protein (active). GTX00294-pro

GTX00294-pro Functional Assay Image

Functional ELISA analysis of GTX00294-pro Mouse S100 beta protein (active) which can bind immobilized RAGE protein.

Mouse S100 beta protein (active). GTX00294-pro

GTX00294-pro Image

SDS-PAGE analysis of GTX00294-pro Mouse S100 beta protein (active).

Mouse S100 beta protein (active). GTX00294-pro

GTX00294-pro Image

WB analysis of GTX00294-pro Mouse S100 beta protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 249