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Mouse SCF protein, His tag (active)

Mouse SCF protein, His tag (active). GTX00323-pro
Mouse SCF protein, His tag (active). GTX00323-pro
Mouse SCF protein, His tag (active). GTX00323-pro

Cat. No. GTX00323-pro

Application

 Functional Assay

Species

 Mouse
Package
10 μg ($299)
See all SCF protein products

APPLICATION

Application Note

Stem cell factor (also known as SCF,  KIT-ligand,  KL, or steel factor) is a cytokine that binds to the c-KIT receptor (CD117). SCF can exist both as a transmembrane protein and a soluble protein. This cytokine plays an important role in hematopoiesis (formation of blood cells), spermatogenesis, and melanogenesis. SCF has been shown to  stimulate the proliferation of Raw264. 7 cells. To test this effect, Raw264. 7 cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach later, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM containing various concentrations of recombinant mouse SCF. After incubated for 120h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of Raw264. 7 cells after incubation with SCF for 120h observed by inverted microscope.

Observed MW

20 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 10mM PBS (pH7.4) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from PBS (pH7.4), 0.01% SKL, 1mM DTT, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Lys24~Ala219 (NP_001334085.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

kit ligand , Clo , Con , Gb , Kitlg , Mgf , SCF , SF , SLF , Sl , blz , contrasted

Database

Research Area

DATA IMAGES

Mouse SCF protein, His tag (active). GTX00323-pro

GTX00323-pro Functional Assay Image

Cell proliferation effect of GTX00323-pro Mouse SCF protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated Raw 264.7 cells cultured in DMEM for 120hrs.
(B) Raw 264.7 cells cultured in DMEM, stimulated with 1 ng/ml SCF for 120hrs.

Mouse SCF protein, His tag (active). GTX00323-pro

GTX00323-pro Image

SDS-PAGE analysis of GTX00323-pro Mouse SCF protein (active).

Mouse SCF protein, His tag (active). GTX00323-pro

GTX00323-pro Image

WB analysis of GTX00323-pro Mouse SCF protein (active).

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SDS
PBS.pdf
Proclin.pdf
Package List Price ($)
$ 299