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Mouse SIRT3 protein, His tag (active)

Mouse SIRT3 protein, His tag (active). GTX00312-pro
Mouse SIRT3 protein, His tag (active). GTX00312-pro
Mouse SIRT3 protein, His tag (active). GTX00312-pro

Cat No. GTX00312-pro

Application

Functional Assay

Species

Mouse
Package
10 μg ($299)

APPLICATION

Application Note

Sirtuin 3 (SIRT3) also known as NAD-dependent deacetylase sirtuin-3 is a member of the mammalian sirtuin family of proteins. SIRT3 is a soluble protein located in the mitochondrial matrix, and contains a mitochondrial processing peptide at the N-terminus. It is a key regulator of succinate dehydrogenase (SDH), which catalyzes the oxidation of succinate to fumarate. Increased succinate concentrations and the specific G protein-coupled receptor 91 (GPR91) are involved in the activation of hepatic stellate cells (HSCs). SIRT3 agonists could help treat metabolic disorders such as obesity, metabolic syndrome and type 2 diabetes. Besides, Dihydrolipoyl Transacetylase (DLAT) has been identified as an interactor of SIRT3, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse SIRT3 and recombinant mouse DLAT. Briefly, SIRT3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to DLAT-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-SITR3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of SIRT3 and DLAT was in a dose dependent manner.

Observed MW

16/29 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Gly5~Arg257 (NP_001120823.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

sirtuin 3 , 2310003L23Rik , AI848213 , Sir2l3

Database

Research Area

DATA IMAGES

Mouse SIRT3 protein, His tag (active). GTX00312-pro

GTX00312-pro Functional Assay Image

Functional ELISA analysis of GTX00312-pro Mouse SIRT3 protein (active) which can bind immobilized DLAT protein.

Mouse SIRT3 protein, His tag (active). GTX00312-pro

GTX00312-pro Image

WB analysis of GTX00312-pro Mouse SIRT3 protein (active).

Mouse SIRT3 protein, His tag (active). GTX00312-pro

GTX00312-pro Image

SDS-PAGE analysis of GTX00312-pro Mouse SIRT3 protein (active).

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 299