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Mouse SIRT3 protein (active)

Mouse SIRT3 protein (active). GTX00312-pro
Mouse SIRT3 protein (active). GTX00312-pro
Mouse SIRT3 protein (active). GTX00312-pro

Cat No. GTX00312-pro

Application Functional Assay
Species Mouse
Package
10 μg ($299)
APPLICATION

Application Note

Sirtuin 3 (SIRT3) also known as NAD-dependent deacetylase sirtuin-3 is a member of the mammalian sirtuin family of proteins. SIRT3 is a soluble protein located in the mitochondrial matrix, and contains a mitochondrial processing peptide at the N-terminus. It is a key regulator of succinate dehydrogenase (SDH), which catalyzes the oxidation of succinate to fumarate. Increased succinate concentrations and the specific G protein-coupled receptor 91 (GPR91) are involved in the activation of hepatic stellate cells (HSCs). SIRT3 agonists could help treat metabolic disorders such as obesity, metabolic syndrome and type 2 diabetes. Besides, Dihydrolipoyl Transacetylase (DLAT) has been identified as an interactor of SIRT3, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse SIRT3 and recombinant mouse DLAT. Briefly, SIRT3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to DLAT-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-SITR3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of SIRT3 and DLAT was in a dose dependent manner.

Observed MW

16/29 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Gly5~Arg257 (NP_001120823.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

sirtuin 3,2310003L23Rik,AI848213,Sir2l3

Database

Research Area

DATA IMAGES
Mouse SIRT3 protein (active). GTX00312-pro

GTX00312-pro Functional Assay Image

Functional ELISA analysis of GTX00312-pro Mouse SIRT3 protein (active) which can bind immobilized DLAT protein.

Mouse SIRT3 protein (active). GTX00312-pro

GTX00312-pro Image

WB analysis of GTX00312-pro Mouse SIRT3 protein (active).

Mouse SIRT3 protein (active). GTX00312-pro

GTX00312-pro Image

SDS-PAGE analysis of GTX00312-pro Mouse SIRT3 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 299