GeneTex
  • Country / Location Selection

United States (US)

Mouse SOCS3 protein (active)

Mouse SOCS3 protein (active). GTX00304-pro
Mouse SOCS3 protein (active). GTX00304-pro
Mouse SOCS3 protein (active). GTX00304-pro

Cat No. GTX00304-pro

Application Functional Assay
Species Mouse
Package
10 μg ($289)
APPLICATION

Application Note

Suppressor of cytokine signaling 3 (SOCS3) is a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family that negatively regulates cytokine signal transduction. SOCS3 is feedback inhibitors of the Janus kinase (JAK) and signal transducer and activator of transcription (STAT) signaling pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. SOCS3 also can bind to JAK2 kinase inhibits its kinase activity and nhibits insulin signaling in adipose tissue and the liver. Besides, Glycoprotein 130 (gp130) has been identified as an interactor of SOCS3, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse SOCS3 and recombinant mouse gp130. Briefly, SOCS3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to SOCS3-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-SOCS3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of SOCS3 and gp130 was in a dose dependent manner.

Observed MW

26 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

Full length protein, N-terminal His-Tag; Met1~Leu225 (NP_031733.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

suppressor of cytokine signaling 3,Cis3,Cish3,EF-10,Ef10,SSI-3,Ssi3

Database

Research Area

DATA IMAGES
Mouse SOCS3 protein (active). GTX00304-pro

GTX00304-pro Functional Assay Image

Functional ELISA analysis of GTX00304-pro Mouse SOCS3 protein (active) which can bind immobilized gp130 protein.

Mouse SOCS3 protein (active). GTX00304-pro

GTX00304-pro Image

SDS-PAGE analysis of GTX00304-pro Mouse SOCS3 protein (active).

Mouse SOCS3 protein (active). GTX00304-pro

GTX00304-pro Image

WB analysis of GTX00304-pro Mouse SOCS3 protein (active).

REFERENCE
There are currently no references for Mouse SOCS3 protein (active) (GTX00304-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Mouse SOCS3 protein (active) (GTX00304-pro). Be the first to share your experience with this product.
Package List Price ($)
$ 289