APPLICATION
Application Note
TNF-a (Tumor necrosis factor), being an endogenous pyrogen, is able to induce fever, apoptotic cell death, inflammation and inhibit tumorigenesis. As reported, TNFa could inhibit the proliferation and induce apoptosis of A549 cells, and the concentration of IL-1β in cell supernatant will increase after stimulation. A549 cells were incubated in DMEM with TNFa (1 ng/ml, 10 ng/ml) for 2h, 4h, 8h, 24h, 48h, then cells were observed by inverted microscope.
Observed MW
21 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.
Preservative
ProClin 300
Storage
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
N-terminal His-Tag; Leu80~Leu235 (NP_001265530.1)
Expression System
E. coli
Purity
> 95%
Endotoxin
< 1 EU/μg
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
tumor necrosis factor , DIF , TNF-a , TNF-alpha , TNFSF2 , TNFalpha , Tnfa , Tnfsf1a , Tnlg1f
Background
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II transmembrane protein and is reported to be cleaved into products that exert distinct biological functions. It plays an important role in the innate immune response as well as regulating homeostasis but is also implicated in diseases of chronic inflammation. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
Database
Research Area
DATA IMAGES
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GTX00287-pro Functional Assay Image
The apoptosis effect of GTX00287-pro Mouse TNF alpha protein (active).
(A) A549 cells cultured in DMEM for 48hrs.
(B) A549 cells cultured in DMEM, stimulated with 1 ng/ml TNF alpha for 48hrs.
(C) A549 cells cultured in DMEM, stimulated with 10 ng/ml TNF alpha for 48hrs.
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GTX00287-pro Functional Assay Image
ELISA detection of secreted IL-1 beta expression from A549 cells stimulated by GTX00287-pro Mouse TNF alpha protein (active) at 1 ng/ml or 10 ng/ml for 48hrs.
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GTX00287-pro Image
SDS-PAGE analysis of GTX00287-pro Mouse TNF alpha protein (active).
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GTX00287-pro Image
WB analysis of GTX00287-pro Mouse TNF alpha protein (active).
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REFERENCE
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REVIEW
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