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Mouse TNF alpha protein, His tag (active)

Mouse TNF alpha protein, His tag (active). GTX67166-pro

Cat. No. GTX67166-pro

Application

 Functional Assay

Species

 Mouse
Package
50 μg ($439)
See all TNF alpha protein products

APPLICATION

Application Note

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is less or equal to 0.02 ng/ml.

Observed MW

18-28 kDa.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

C-terminal His-Tag; Length: 162 a.a. Sequence: LRSSSQNSSD KPVAHVVANH QVEEQLEWLS QRANALLANG MDLKDNQLVV PADGLYLVYS QVLFKGQGCP DYVLLTHTVS RFAISYQEKV NLLSAVKSPC PKDTPEGAEL KPWYEPIYLG GVFQLEKGDQ LSAEVNLPKY LDFAESGQVY FGVIALHHHH HH

Expression System

Baculovirus

Purity

> 90% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor necrosis factor , DIF , TNF-a , TNF-alpha , TNFSF2 , TNFalpha , Tnfa , Tnfsf1a , Tnlg1f

Background

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II transmembrane protein and is reported to be cleaved into products that exert distinct biological functions. It plays an important role in the innate immune response as well as regulating homeostasis but is also implicated in diseases of chronic inflammation. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Database

Research Area

DATA IMAGES

Mouse TNF alpha protein, His tag (active). GTX67166-pro

GTX67166-pro Image

3 μg of GTX67166-pro Mouse TNF alpha protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

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SDS
PBS.pdf
Package List Price ($)
$ 439