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Mouse Tyrosine Aminotransferase protein (active)

Mouse Tyrosine Aminotransferase protein (active). GTX00309-pro
Mouse Tyrosine Aminotransferase protein (active). GTX00309-pro
Mouse Tyrosine Aminotransferase protein (active). GTX00309-pro

Cat No. GTX00309-pro

Application Functional Assay
Species Mouse
Package
10 μg ($279)
APPLICATION

Application Note

Tyrosine aminotransferase (TAT) is an enzyme present in the liver and catalyzes the conversion of tyrosine to 4-hydroxyphenylpyruvate. In humans, the tyrosine aminotransferase protein is encoded by the TAT gene. A deficiency of the enzyme in humans can result in what is known as Type II Tyrosinemia, wherein there is an abundance of tyrosine as a result of tyrosine failing to undergo an aminotransferase reaction to form 4-hydroxyphenylpyruvate. Besides, Glutamine synthetase (GS) has been identified as an interactor of TAT, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse TAT and recombinant mouse GS. Briefly, TAT were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to GS-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-TATpAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of TAT and GS was in a dose dependent manner.

Observed MW

34 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Pro190~Lys454 (NP_666326.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

tyrosine aminotransferase

Background

This gene encodes a liver-specific mitochondrial enzyme that catalyzes the conversion of L-tyrosine into p-hydroxyphenylpyruvate. Regulated by glucocorticoid and polypeptide hormones, this gene's expression is affected by deletion of a regulatory region near the albino locus on chromosome 7. Mutations in this gene cause tyrosinemia type II in humans. [provided by RefSeq, Mar 2010]

Database

DATA IMAGES
Mouse Tyrosine Aminotransferase protein (active). GTX00309-pro

GTX00309-pro Functional Assay Image

Functional ELISA analysis of GTX00309-pro Mouse Tyrosine Aminotransferase protein (active) which can bind immobilized GS protein.

Mouse Tyrosine Aminotransferase protein (active). GTX00309-pro

GTX00309-pro Image

WB analysis of GTX00309-pro Mouse Tyrosine Aminotransferase protein (active).

Mouse Tyrosine Aminotransferase protein (active). GTX00309-pro

GTX00309-pro Image

SDS-PAGE analysis of GTX00309-pro Mouse Tyrosine Aminotransferase protein (active).

REFERENCE
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Package List Price ($)
$ 279