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Mouse UCHL3 protein, His tag (active)

Mouse UCHL3 protein, His tag (active). GTX67179-pro

Cat No. GTX67179-pro

Application

Functional Assay

Species

Mouse
Package
10 μg ($219)

APPLICATION

Application Note

Specific activity is > 9000 pmol/min/μg, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of ubiquitin-AMC per minute at pH 7.5, at 37ºC.

Observed MW

28-40 kDa.

PROPERTIES

Form

Liquid

Buffer

PBS (pH 7.4) with 10% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml

Region/Sequence

Full length protein, C-terminal His-Tag; Length: 238 a.a. Sequence: MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMEPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERAKFLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGKTSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA LEHHHHHH

Expression System

Baculovirus

Purity

> 90% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)

Database

Research Area

DATA IMAGES

Mouse UCHL3 protein, His tag (active). GTX67179-pro

GTX67179-pro Image

3 μg of GTX67179-pro Mouse UCHL3 protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 219