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Mpox virus A29L antibody [HL2527]

Recombinant antibody
Anti-Mpox virus A29L antibody [HL2527] used in Western Blot (WB). GTX638888
Anti-Mpox virus A29L antibody [HL2527] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638888
Anti-Mpox virus A29L antibody [HL2527] used in Western Blot (WB). GTX638888
Anti-Mpox virus A29L antibody [HL2527] used in Western Blot (WB). GTX638888

Cat. No. GTX638888

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2527

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Monkeypox, Vaccinia virus
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Monkeypox

Immunogen

Full length of Monkeypox virus A29L recombinant protein (MPXV_USA_2022_MA001)

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-Mpox virus A29L antibody [HL2527] used in Western Blot (WB). GTX638888

GTX638888 WB Image

Non-infected (–) and infected (+) BSC-40 whole cell extracts (5 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Mpox virus A29L antibody [HL2527] (GTX638888) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Mpox virus A29L antibody [HL2527] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638888

GTX638888 ICC/IF Image

Mpox virus A29L antibody [HL2527] detects Mpox virus A29L protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Mpox virus A29L stained by Mpox virus A29L antibody [HL2527] (GTX638888) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Mpox virus A29L antibody [HL2527] used in Western Blot (WB). GTX638888

GTX638888 WB Image

Non-infected (–) and infected (+) BSC-40 whole cell extracts were separated by 15% SDS-PAGE, and the membrane was blotted with Mpox virus A29L antibody [HL2527] (GTX638888) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Mpox virus A29L antibody [HL2527] used in Western Blot (WB). GTX638888

GTX638888 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Mpox virus A29L antibody [HL2527] (GTX638888) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations