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Mpx antibody

Orthogonal
Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379
Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379
Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379
Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379
Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379
Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379
Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379

Cat. No. GTX128379

Reference 22
5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P, IHC-Fr, IHC-Wm, IHC

Reactivity

 Zebrafish, Atlantic salmon
Package
100 μl ($399),
25 μl ($169)
See all Mpx products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P Assay dependent
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish Mpx. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885768

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myeloid-specific peroxidase , drf , fj80f04 , mpo , wu:fj80f04

Database

Research Area

DATA IMAGES

Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379

GTX128379 IHC-Wm Image

Mpx antibody detects Mpx protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Mpx antibody (GTX128379) dilution: 1:100.
Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379

GTX128379 IHC-Wm Image

Mpx antibody detects Mpx protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 3 days-post-fertilization zebrafish embryo.
Green: Mpx stained by Mpx antibody (GTX128379) diluted at 1:100.

Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379

GTX128379 IHC-Wm Image

Mpx antibody detects Mpx antibody protein at leukocyte on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
Mpx antibody (GTX128379) dilution: 1:200.
Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379

GTX128379 IHC-Wm Image

Mpx antibody detects Mpx protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Mpx stained by Mpx antibody (GTX128379) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379

GTX128379 IHC-Wm Image

Mpx antibody detects Mpx protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Mpx stained by Mpx antibody (GTX128379) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379

GTX128379 IHC-Wm Image

Mpx antibody detects Mpx protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Mpx stained by Mpx antibody (GTX128379) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379

GTX128379 IHC-Wm Image

Mpx antibody detects Mpx protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Mpx stained by Mpx antibody (GTX128379) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

REFERENCE

Application Reference

Wei KH et al. Elife 2023; 12 Comparative single-cell profiling reveals distinct cardiac resident macrophages essential for zebrafish heart regeneration.
Opri?oreanu AM et al. Theranostics 2023; 13 (8) : 2531-2551 Drug screening in zebrafish larvae reveals inflammation-related modulators of secondary damage after spinal cord injury in mice.
M El Ma? et al. Nat Aging 2023; Gut-specific telomerase expression counteracts systemic aging in telomerase-deficient zebrafish
Application : IHC-Fr
Reactivity : Zebrafish
Kehua Chen et al. Biomedicines 2022; 10 (2) Loss of atm in Zebrafish as a Model of Ataxia-Telangiectasia Syndrome.
Application : IHC-P
Reactivity : Zebrafish
Isles HM et al. Elife 2021; 10 Pioneer neutrophils release chromatin within in vivo swarms.
Reactivity : Zebrafish
Show More +
Submit a Reference

REVIEW

Mpx antibody Cat. No. GTX128379

Rating
( Average 5 based on 5 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 2 users reviews)
IHC (Whole mount)(IHC-Wm)
( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC)
( Average 5 based on 2 users reviews)
Anti-Mpx antibody used in Immunohistochemistry (IHC). GTX128379
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018. PMID: 30143654
Anti-Mpx antibody used in Immunohistochemistry (IHC). GTX128379
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018. PMID: 30143654
Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Wm
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017. PMID: 28747644
Anti-Mpx antibody used in IHC (Paraffin sections) (IHC-P). GTX128379
Date : Anonymous submitted on 23-May-2016
Score :
Application Tested : IHC-P
Sample Species : Atlantic salmon
Sample : stomach tissue
Blocking : 5% BSA, 25°C, 1Hr
Fixation : 10% formalin
Primary Antibody : 1:200, 4°C, 16Hr
Anti-Mpx antibody used in IHC (Paraffin sections) (IHC-P). GTX128379
Date : Anonymous submitted on 23-May-2016
Score :
Application Tested : IHC-P
Sample Species : Atlantic salmon
Sample : Headkidney tissue
Blocking : 5% BSA, 25°C, 1Hr
Fixation : 10% formalin
Primary Antibody : 1:200, 4°C, 16Hr
Submit a Review
Application Reference
Wei KH et al. Elife 2023; 12 Comparative single-cell profiling reveals distinct cardiac resident macrophages essential for zebrafish heart regeneration.
Opri?oreanu AM et al. Theranostics 2023; 13 (8):2531-2551 Drug screening in zebrafish larvae reveals inflammation-related modulators of secondary damage after spinal cord injury in mice.
M El Ma? et al. Nat Aging 2023; Gut-specific telomerase expression counteracts systemic aging in telomerase-deficient zebrafish
Application : IHC-Fr
Reactivity : Zebrafish
Kehua Chen et al. Biomedicines 2022; 10 (2) Loss of atm in Zebrafish as a Model of Ataxia-Telangiectasia Syndrome.
Application : IHC-P
Reactivity : Zebrafish
Isles HM et al. Elife 2021; 10 Pioneer neutrophils release chromatin within in vivo swarms.
Reactivity : Zebrafish
Keatinge M et al. PLoS Genet 2021; 17 (4):e1009515 CRISPR gRNA phenotypic screening in zebrafish reveals pro-regenerative genes in spinal cord injury.
Application : IHC-Wm
Reactivity : Zebrafish
P?voa V et al. Nat Commun 2021; 12 (1):1156 Innate immune evasion revealed in a colorectal zebrafish xenograft model.
Application : IHC-Wm
Reactivity : Zebrafish
Bise T et al. Sci Rep 2020; 10 (1):11551 Multiple cryoinjuries modulate the efficiency of zebrafish heart regeneration.
Castro CN et al. J Exp Med 2020; 217 (12) NCKAP1L defects lead to a novel syndrome combining immunodeficiency, lymphoproliferation, and hyperinflammation.
Application : IHC-Wm
Reactivity : Zebrafish
Solis CJ et al. Front Immunol 2020; 11:1330 Intestinal Inflammation Induced by Soybean Meal Ingestion Increases Intestinal Permeability and Neutrophil Turnover Independently of Microbiota in Zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Ong SLM et al. Sci Rep 2020; 10 (1):11801 Microfibril-associated glycoprotein 4 (Mfap4) regulates haematopoiesis in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Marcus Keatinge et al. bioRxiv 2020; (Epub) Phenotypic screening using synthetic CRISPR gRNAs reveals pro-regenerative genes in spinal cord injury.
Application : IHC-Wm
Reactivity : Zebrafish
Harrison MR et al. Elife 2019; 8 Late developing cardiac lymphatic vasculature supports adult zebrafish heart function and regeneration.
Coronado M et al. Front Immunol 2019; 10:610 Soybean Meal-Induced Intestinal Inflammation in Zebrafish Is T Cell-Dependent and Has a Th17 Cytokine Profile.
Novoa B et al. Aging Cell 2019;:e13020 Rag1 immunodeficiency-induced early aging and senescence in zebrafish are dependent on chronic inflammation and oxidative stress.
Application : IHC
Reactivity : Zebrafish
Kenyon A et al. Dis Model Mech 2018; 11 (4) Generation of a double binary transgenic zebrafish model to study myeloid gene regulation in response to oncogene activation in melanocytes.
Lopez-Munoz A et al. Sci Rep 2018; 8 (1):12802 An Adult Zebrafish Model Reveals that Mucormycosis Induces Apoptosis of Infected Macrophages.
Application : IHC-P
Reactivity : Zebrafish
Themistoklis M. Tsarouchas et al. bioRxiv 2018; 2018 (Epub) Dynamic control of proinflammatory cytokines Il-1β and Tnf-α by macrophages is necessary for functional spinal cord regeneration in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Mitchell DM et al. J Neuroinflammation 2018; 15 (1):163 Dynamic changes in microglial and macrophage characteristics during degeneration and regeneration of the zebrafish retina.
Application : IHC-Fr
Reactivity : Zebrafish
Kenyon A et al. Dis. Models Mech. 2018; (Epub) Generation of a double binary transgenic zebrafish model to study myeloid gene regulation in response to oncogene activation in melanocytes.
Application : IHC-Wm
Reactivity : Zebrafish
Carapito R et al. J Clin Invest 2017; (Epub) Mutations in signal recognition particle SRP54 cause syndromic neutropenia with Shwachman-Diamond-like features.
Application : IHC-Wm
Reactivity : Zebrafish
Kenyon A et al. Sci Rep 2017; 7 (1):6505 Active nuclear transcriptome analysis reveals inflammasome-dependent mechanism for early neutrophil response to Mycobacterium marinum.
Application : IHC-Wm
Reactivity : Zebrafish
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169