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Mpx antibody

Orthogonal
Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379
Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379
Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379
Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379
Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379
Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379
Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379

Cat No. GTX128379

Reference 20
5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P, IHC-Fr, IHC-Wm, IHC

Reactivity

 Zebrafish, Atlantic salmon
Package
200 μl ($409),
50 μl ($219)
See all zebrafish Mpx products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P Assay dependent
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

1XPBS,20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish Mpx. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885768

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

myeloid-specific peroxidase , drf , fj80f04 , mpo , wu:fj80f04

Database

Research Area

DATA IMAGES

Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379

GTX128379 IHC-Wm Image

Mpx antibody detects Mpx protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Mpx antibody (GTX128379) dilution: 1:100.
Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379

GTX128379 IHC-Wm Image

Mpx antibody detects Mpx protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Mpx stained by Mpx antibody (GTX128379) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379

GTX128379 IHC-Wm Image

Mpx antibody detects Mpx protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 3 days-post-fertilization zebrafish embryo.
Green: Mpx stained by Mpx antibody (GTX128379) diluted at 1:100.

Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379

GTX128379 IHC-Wm Image

Mpx antibody detects Mpx antibody protein at leukocyte on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
Mpx antibody (GTX128379) dilution: 1:200.
Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379

GTX128379 IHC-Wm Image

Mpx antibody detects Mpx protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Mpx stained by Mpx antibody (GTX128379) diluted at 1:100.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379

GTX128379 IHC-Wm Image

Mpx antibody detects Mpx protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Mpx stained by Mpx antibody (GTX128379) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379

GTX128379 IHC-Wm Image

Mpx antibody detects Mpx protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Mpx stained by Mpx antibody (GTX128379) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

REFERENCE

Application Reference

Isles HM et al. Elife 2021; 10 Pioneer neutrophils release chromatin within in vivo swarms.
Reactivity : Zebrafish
Keatinge M et al. PLoS Genet 2021; 17 (4) : e1009515 CRISPR gRNA phenotypic screening in zebrafish reveals pro-regenerative genes in spinal cord injury.
Application : IHC-Wm
Reactivity : Zebrafish
P?voa V et al. Nat Commun 2021; 12 (1) : 1156 Innate immune evasion revealed in a colorectal zebrafish xenograft model.
Application : IHC-Wm
Reactivity : Zebrafish
Castro CN et al. J Exp Med 2020; 217 (12) NCKAP1L defects lead to a novel syndrome combining immunodeficiency, lymphoproliferation, and hyperinflammation.
Bise T et al. Sci Rep 2020; 10 (1) : 11551 Multiple cryoinjuries modulate the efficiency of zebrafish heart regeneration.
Show More +
Submit a Reference

REVIEW

Mpx antibody Cat No. GTX128379

Rating
( Average 5 based on 5 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 2 users reviews)
IHC (Whole mount)(IHC-Wm)
( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC)
( Average 5 based on 2 users reviews)
Anti-Mpx antibody used in Immunohistochemistry (IHC). GTX128379
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 30143654
Anti-Mpx antibody used in Immunohistochemistry (IHC). GTX128379
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 30143654
Anti-Mpx antibody used in IHC (Whole mount) (IHC-Wm). GTX128379
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Wm
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28747644
Anti-Mpx antibody used in IHC (Paraffin sections) (IHC-P). GTX128379
Date : Anonymous submitted on 23-May-2016
Score :
Application Tested : IHC-P
Sample Species : Atlantic salmon
Sample : stomach tissue
Blocking : 5% BSA, 25°C, 1Hr
Fixation : 10% formalin
Primary Antibody : 1:200, 4°C, 16Hr
Anti-Mpx antibody used in IHC (Paraffin sections) (IHC-P). GTX128379
Date : Anonymous submitted on 23-May-2016
Score :
Application Tested : IHC-P
Sample Species : Atlantic salmon
Sample : Headkidney tissue
Blocking : 5% BSA, 25°C, 1Hr
Fixation : 10% formalin
Primary Antibody : 1:200, 4°C, 16Hr
Submit a Review
Application Reference
Isles HM et al. Elife 2021; 10 Pioneer neutrophils release chromatin within in vivo swarms.
Reactivity : Zebrafish
Keatinge M et al. PLoS Genet 2021; 17 (4):e1009515 CRISPR gRNA phenotypic screening in zebrafish reveals pro-regenerative genes in spinal cord injury.
Application : IHC-Wm
Reactivity : Zebrafish
P?voa V et al. Nat Commun 2021; 12 (1):1156 Innate immune evasion revealed in a colorectal zebrafish xenograft model.
Application : IHC-Wm
Reactivity : Zebrafish
Castro CN et al. J Exp Med 2020; 217 (12) NCKAP1L defects lead to a novel syndrome combining immunodeficiency, lymphoproliferation, and hyperinflammation.
Bise T et al. Sci Rep 2020; 10 (1):11551 Multiple cryoinjuries modulate the efficiency of zebrafish heart regeneration.
Castro CN et al. J Exp Med 2020; 217 (12) NCKAP1L defects lead to a novel syndrome combining immunodeficiency, lymphoproliferation, and hyperinflammation.
Application : IHC-Wm
Reactivity : Zebrafish
Solis CJ et al. Front Immunol 2020; 11:1330 Intestinal Inflammation Induced by Soybean Meal Ingestion Increases Intestinal Permeability and Neutrophil Turnover Independently of Microbiota in Zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Ong SLM et al. Sci Rep 2020; 10 (1):11801 Microfibril-associated glycoprotein 4 (Mfap4) regulates haematopoiesis in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Solis CJ et al. Front Immunol 2020; 11:1330 Intestinal Inflammation Induced by Soybean Meal Ingestion Increases Intestinal Permeability and Neutrophil Turnover Independently of Microbiota in Zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Marcus Keatinge et al. bioRxiv 2020; (Epub) Phenotypic screening using synthetic CRISPR gRNAs reveals pro-regenerative genes in spinal cord injury.
Application : IHC-Wm
Reactivity : Zebrafish
Harrison MR et al. Elife 2019; 8 Late developing cardiac lymphatic vasculature supports adult zebrafish heart function and regeneration.
Coronado M et al. Front Immunol 2019; 10:610 Soybean Meal-Induced Intestinal Inflammation in Zebrafish Is T Cell-Dependent and Has a Th17 Cytokine Profile.
Novoa B et al. Aging Cell 2019;:e13020 Rag1 immunodeficiency-induced early aging and senescence in zebrafish are dependent on chronic inflammation and oxidative stress.
Application : IHC
Reactivity : Zebrafish
Kenyon A et al. Dis Model Mech 2018; 11 (4) Generation of a double binary transgenic zebrafish model to study myeloid gene regulation in response to oncogene activation in melanocytes.
Lopez-Munoz A et al. Sci Rep 2018; 8 (1):12802 An Adult Zebrafish Model Reveals that Mucormycosis Induces Apoptosis of Infected Macrophages.
Application : IHC-P
Reactivity : Zebrafish
Themistoklis M. Tsarouchas et al. bioRxiv 2018; 2018 (Epub) Dynamic control of proinflammatory cytokines Il-1β and Tnf-α by macrophages is necessary for functional spinal cord regeneration in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Mitchell DM et al. J Neuroinflammation 2018; 15 (1):163 Dynamic changes in microglial and macrophage characteristics during degeneration and regeneration of the zebrafish retina.
Application : IHC-Fr
Reactivity : Zebrafish
Kenyon A et al. Dis. Models Mech. 2018; (Epub) Generation of a double binary transgenic zebrafish model to study myeloid gene regulation in response to oncogene activation in melanocytes.
Application : IHC-Wm
Reactivity : Zebrafish
Carapito R et al. J Clin Invest 2017; (Epub) Mutations in signal recognition particle SRP54 cause syndromic neutropenia with Shwachman-Diamond-like features.
Application : IHC-Wm
Reactivity : Zebrafish
Kenyon A et al. Sci Rep 2017; 7 (1):6505 Active nuclear transcriptome analysis reveals inflammasome-dependent mechanism for early neutrophil response to Mycobacterium marinum.
Application : IHC-Wm
Reactivity : Zebrafish
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 409
$ 219