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Mre11 antibody [12D7]

KO/KD Comparison Orthogonal
Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Anti-Mre11 antibody [12D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70212
Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Anti-Mre11 antibody [12D7] used in IHC (Paraffin sections) (IHC-P). GTX70212

Cat No. GTX70212

Reference 154
14

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 12D7

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP, ELISA, Functional Assay, PLA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329)
See all Mre11 products
PRODUCT

Summary

MRE11 antibody recognizes MRE11 protein, an enzyme with a predicted molecular weight of ~81 kDa. MRE11 forms the core of the MRN (MRE11-RAD50-NBS1) complex, one of the first components of the DNA damage response (DDR) to DNA double-strand breaks where it recruits other signaling factors required for repair. MRE11 has both exonuclease and endonuclease activities, and is responsible for the DNA binding of the complex.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP Assay dependent
ELISA Assay dependent
Functional Assay Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

HeLa , Jurkat , Raji , NCI-H929 , 293T , human Mre11-transfected 293T , PC-12

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 182-582 of Mre11 expressed in E. coli.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_372398

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MRE11 homolog, double strand break repair nuclease , ATLD , HNGS1 , MRE11A , MRE11B

Cellular Localization

Nucleus

Background

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212

GTX70212 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (GTX70212) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212

GTX70212 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Mre11 antibody [12D7] (GTX70212) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212

GTX70212 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (GTX70212) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212

GTX70212 WB Image

Mre11 antibody [12D7] detects Mre11 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of human Mre11-transfected 293T cells
7.5% SDS-PAGE
Mre11 antibody [12D7] (GTX70212) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Mre11 antibody [12D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70212

GTX70212 ICC/IF Image

Mre11 antibody [12D7] detects Mre11 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Mre11 stained by Mre11 antibody [12D7] (GTX70212) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212

GTX70212 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (GTX70212) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212

GTX70212 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (GTX70212) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Mre11 antibody [12D7] used in IHC (Paraffin sections) (IHC-P). GTX70212

GTX70212 IHC-P Image

Mre11 antibody [12D7] detects Mre11 protein at nucleus by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human colon cancer.
Mre11 stained by Mre11 antibody [12D7] (GTX70212) diluted at 1:300.
Antigen Retrieval: EDTA buffer, 20 min

REFERENCE

Application Reference

Xu X et al. Nat Commun 2021; 12 (1) : 3520 The epigenetic regulator LSH maintains fork protection and genomic stability via MacroH2A deposition and RAD51 filament formation.
Application : WB
Reactivity : Human
Dubich T et al. J Mol Med (Berl) 2021; 99 (3) : 425-438 3D culture conditions support Kaposi's sarcoma herpesvirus (KSHV) maintenance and viral spread in endothelial cells.
Application : WB
Reactivity : Human
Suzuki R et al. Nucleic Acids Res 2021; 49 (1) : 244-256 The fragility of a structurally diverse duplication block triggers recurrent genomic amplification.
Application : ICC/IF
Reactivity : Human
Yamashita R et al. Mutat Res 2020; 858-860 : 503273 Cell line-dependent difference in glutathione levels affects the cigarette sidestream smoke-induced inhibition of nucleotide excision repair.
Application : WB
Reactivity : Human
Bennett LG et al. Sci Adv 2020; 6 (28) : eaba5974 MRNIP is a replication fork protection factor.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

Mre11 antibody [12D7] Cat No. GTX70212

Rating
( Average 5 based on 14 users reviews)
Application
Western Blot(WB)
( Average 5 based on 8 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 5 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Date : Anonymous submitted on 18-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : HaCaT cells
Amount used : 4 μg
Blocking : Skim milk, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, 11Hr
Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Open Biol in 2015.PMID: 25924630
Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 25826455
Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017.PMID: 29042561
Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal BMC Cancer in 2014.PMID: 24928521
Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017.PMID: 29042561
Anti-Mre11 antibody [12D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70212
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Open Biol in 2015.PMID: 25924630
Anti-Mre11 antibody [12D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70212
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Virol in 2017.PMID: 28855246
Anti-Mre11 antibody [12D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70212
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Viruses in 2015.PMID: 26057167
Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2010.PMID: 20949077
Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal BMC Biol in 2015.PMID: 25857663
Anti-Mre11 antibody [12D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70212
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nucleic Acids Res in 2016.PMID: 26446986
Anti-Mre11 antibody [12D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70212
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nucleic Acids Res in 2016.PMID: 26446986
Anti-Mre11 antibody [12D7] used in IHC (Paraffin sections) (IHC-P). GTX70212
Date : Anonymous submitted on 21-Oct-2020
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : colon cancer tissue
Fixation : EDTA buffer, 0.3°C
Primary Antibody : 1:300, 4°C, 16Hr
Show More +
Submit a Review
Application Reference
Xu X et al. Nat Commun 2021; 12 (1):3520 The epigenetic regulator LSH maintains fork protection and genomic stability via MacroH2A deposition and RAD51 filament formation.
Application : WB
Reactivity : Human
Dubich T et al. J Mol Med (Berl) 2021; 99 (3):425-438 3D culture conditions support Kaposi's sarcoma herpesvirus (KSHV) maintenance and viral spread in endothelial cells.
Application : WB
Reactivity : Human
Suzuki R et al. Nucleic Acids Res 2021; 49 (1):244-256 The fragility of a structurally diverse duplication block triggers recurrent genomic amplification.
Application : ICC/IF
Reactivity : Human
Yamashita R et al. Mutat Res 2020; 858-860:503273 Cell line-dependent difference in glutathione levels affects the cigarette sidestream smoke-induced inhibition of nucleotide excision repair.
Application : WB
Reactivity : Human
Bennett LG et al. Sci Adv 2020; 6 (28):eaba5974 MRNIP is a replication fork protection factor.
Application : WB
Reactivity : Human
Duan H et al. J Cell Biol 2020; 219 (6) E3 ligase RFWD3 is a novel modulator of stalled fork stability in BRCA2-deficient cells.
Qi F et al. J Radiat Res 2020; 61 (3):368-375 FXR1 is a novel MRE11-binding partner and participates in oxidative stress responses.
Application : WB
Reactivity : Human
Ragamin A et al. Am J Med Genet A 2020; 182 (6):1378-1386 Human RAD50 deficiency: Confirmation of a distinctive phenotype.
Application : WB
Reactivity : Human
Völkening L et al. FASEB J 2020; 34 (2):2812-2820 RAD50 regulates mitotic progression independent of DNA repair functions.
Application : WB
Reactivity : Human
Mooser C et al. Nat Commun 2020; 11 (1):123 Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation.
Application : ICC/IF
Reactivity : Human
Lorenson MY et al. Biol Methods Protoc 2019; 4 (1):bpy014 Enzyme-linked oligonucleotide hybridization assay for direct oligo measurement in blood.
Murata MM et al. Mol Biol Cell 2019; 30 (20):2584-2597 NAD+ consumption by PARP1 in response to DNA damage triggers metabolic shift critical for damaged cell survival.
Ly P et al. Nat Genet 2019; 51 (4):705-715 Chromosome segregation errors generate a diverse spectrum of simple and complex genomic rearrangements.
Godau J et al. DNA Repair (Amst) 2019; 77:96-108 Identification of a miniature Sae2/Ctp1/CtIP ortholog from Paramecium tetraurelia required for sexual reproduction and DNA double-strand break repair.
Sohn SY et al. MBio 2019; 10 (1):pii: e00022-19 Mechanism of Adenovirus E4-ORF3-Mediated SUMO Modifications.
Application : WB
Reactivity : Human
Mary Y Lorenson et al. Biology Methods and Protocols 2019; 4 (1) Enzyme-linked oligonucleotide hybridization assay for direct oligo measurement in blood.
Burger K et al. Nucleic Acids Res 2019; (Epub) Tyrosine kinase c-Abl couples RNA polymerase II transcription to DNA double-strand breaks.
Yizhou Joseph He et al. Nature 2018; (Epub) DYNLL1 binds to MRE11 to limit DNA end resection in BRCA1-deficient cells.
Application : WB, IP
Reactivity : Human
Przetocka S et al. Mol Cell 2018; 72 (3):568-582.e6 CtIP-Mediated Fork Protection Synergizes with BRCA1 to Suppress Genomic Instability upon DNA Replication Stress.
Application : WB
Reactivity : Human
Fujiwara C et al. Sci Rep 2018; 8 (1):14827 Cell-based chemical fingerprinting identifies telomeres and lamin A as modifiers of DNA damage response in cancer cells.
Application : WB
Reactivity : Human
Michael Murata et al. bioRxiv 2018; (Epub) NAD consumption by PARP1 in response to DNA damage triggers metabolic shift critical for damaged cell survival.
Application : ICC/IF
Reactivity : Human
Johnson TE et al. Cell Rep 2018; 24 (6):1471-1483 Homeodomain Proteins Directly Regulate ATM Kinase Activity.
Application : WB
Reactivity : Human
Cai M et al. Int J Cancer 2018; (Epub) inhibiting homologous recombination decreases extrachromosomal amplification but has no effect on intrachromosomal amplification in *-resistant colon cancer cells.
Application : WB
Reactivity : Human
Shou J et al. Mol Cell 2018; 71 (4):498-509.e4 Precise and Predictable CRISPR Chromosomal Rearrangements Reveal Principles of Cas9-Mediated Nucleotide Insertion.
Application : WB
Reactivity : Human
Kong X et al. bioRxiv 2018; (Epub) Biphasic recruitment of TRF2 to DNA damage sites promotes non-sister chromatid homologous recombination repair.
Application : ICC/IF
Reactivity : Human
Park S et al. Mol Cancer Res 2018; (Epub) Therapeutic Effect of Quinacrine, an Anti-protozoan Drug, by Selective Suppression of p-CHK1/2 in p53-negative Malignant Cancers.
Application : WB
Reactivity : Human
Ahmed KM et al. Mol Cell Biol 2018; (Epub) β1-integrin impacts Rad51 stability and DNA double-strand break repair by homologous recombination.
Application : WB
Reactivity : Human
Hollingworth R et al. J Virol 2017; 91 (22) Localization of Double-Strand Break Repair Proteins to Viral Replication Compartments following Lytic Reactivation of Kaposi's Sarcoma-Associated Herpesvirus.
Mujoo K et al. Stem Cell Reports 2017; 9 (5):1660-1674 Differentiation of Human Induced Pluripotent or Embryonic Stem Cells Decreases the DNA Damage Repair by Homologous Recombination.
Application : ICC/IF
Reactivity : Human
Taglialatela A et al. Mol Cell 2017; 68 (2):414-430.e8 Restoration of Replication Fork Stability in BRCA1- and BRCA2-Deficient Cells by Inactivation of SNF2-Family Fork Remodelers.
Application : PLA
Reactivity : Human
Lou J et al. Nat Commun 2017; 8 (1):985 AUNIP/C1orf135 directs DNA double-strand breaks towards the homologous recombination repair pathway.
Application : WB
Reactivity : Human
Henrich SM et al. Sci Rep 2017; 7:41663 Interplay with the Mre11-Rad50-Nbs1 complex and phosphorylation by GSK3β implicate human B-Myb in DNA-damage signaling.
Application : WB
Reactivity : Human
Smith EA et al. Sci Rep 2017; 7:44662 DEK is required for homologous recombination repair of DNA breaks.
Application : WB
Reactivity : Human
Mattoo AR et al. Mol Cell Biol 2016; (Epub) MCL-1 depletion impairs DNA DSB repair and re-initiation of stalled DNA replication forks.
Application : WB
Reactivity : Human
Johnson BA et al. Virology 2016; 500:22-34 The Rb binding domain of HPV31 E7 is required to maintain high levels of DNA repair factors in infected cells.
Application : WB
Reactivity : Human
Lou DI et al. Cell Host Microbe 2016; 20 (2):178-88 An Intrinsically Disordered Region of the DNA Repair Protein Nbs1 Is a Species-Specific Barrier to Herpes Simplex Virus 1 in Primates.
Application : WB
Reactivity : Human
Renaud E et al. Nucleic Acids Res 2016; 44 (2):648-56 Impaired TIP60-mediated H4K16 acetylation accounts for the aberrant chromatin accumulation of 53BP1 and RAP80 in Fanconi anemia pathway-deficient cells.
Bowen C et al. Cancer Res 2015; 75 (13):2686-98 NKX3.1 Suppresses TMPRSS2-ERG Gene Rearrangement and Mediates Repair of Androgen Receptor-Induced DNA Damage.
Application : WB
Reactivity : Human
Markiewicz E et al. Open Biol 2015; 5 (4):150011 Nonlinear ionizing radiation-induced changes in eye lens cell proliferation, cyclin D1 expression and lens shape.
Application : WB
Reactivity : Human
Kozlov SV et al. Mol Cell Proteomics 2015; (Epub) ROS-activated ATM-dependent phosphorylation of cytoplasmic substrates identified by large scale phosphoproteomics screen.
Application : WB
Reactivity : Human
Kijas AW et al. Nucleic Acids Res 2015; 43 (17):8352-67 ATM-dependent phosphorylation of MRE11 controls extent of resection during homology directed repair by signalling through Exonuclease 1.
Application : WB
Reactivity : Human
Hollingworth R et al. Viruses 2015; 7 (6):2908-27 Activation of DNA Damage Response Pathways during Lytic Replication of KSHV.
Application : ICC/IF
Reactivity : Human
Nakajima NI et al. PLoS One 2015; 10 (3):e0122582 Pre-exposure to ionizing radiation stimulates DNA double strand break end resection, promoting the use of homologous recombination repair.
Application : WB, ICC/IF
Reactivity : Human
Harten SK et al. BMC Biol 2015; 13:21 The recently identified modifier of murine metastable epialleles, Rearranged L-Myc Fusion, is involved in maintaining epigenetic marks at CpG island shores and enhancers.
Application : WB
Reactivity : Mouse
Huang YL et al. Hum Mol Genet 2015; 24:3506-17 FGFR2 regulates Mre11 expression and double-strand break repair via the MEK-ERK-POU1F1 pathway in breast tumorigenesis.
Application : WB
Reactivity : Human
Schroder-Heurich B et al. BMC Cancer 2014; 14:434 Functional deficiency of NBN, the Nijmegen breakage syndrome protein, in a p.R215W mutant breast cancer cell line.
Application : WB
Reactivity : Human
Pathania S et al. Nat Commun 2014; 5:5496 BRCA1 haploinsufficiency for replication stress suppression in primary cells.
Application : ICC/IF
Reactivity : Human
Jian W et al. Anticancer Drugs 2014; Activity of CEP-9722, a poly (ADP-ribose) polymerase inhibitor, in urothelial carcinoma correlates inversely with homologous recombination repair response to DNA damage.
Application : WB
Reactivity : Human
Zhou Y et al. Nucleic Acids Res 2014; 42 (3):e19 Quantitation of DNA double-strand break resection intermediates in human cells.
Application : WB
Reactivity : Human
Schroder-Heurich B et al. FASEB J 2014; 28 (3):1331-41 Protective role of RAD50 on chromatin bridges during abnormal cytokinesis.
Application : ICC/IF
Kong X et al. Mol Cell Biol 2014; 34 (4):685-98 Distinct Functions of Human Cohesin-SA1 and Cohesin-SA2 in Double-Strand Break Repair.
Application : WB
O'Sullivan RJ et al. Nat Struct Mol Biol 2014; 21 (2):167-74 Rapid induction of alternative lengthening of telomeres by depletion of the histone chaperone ASF1.
Application : WB
Reactivity : Human
Simoes M et al. Vet Microbiol 2013; 165 (43102):140-7 Host DNA damage response facilitates African swine fever virus infection.
Application : WB
Lee JH et al. J Biol Chem 2013; 288 (18):12840-51 Ataxia telangiectasia-mutated (ATM) kinase activity is regulated by ATP-driven conformational changes in the Mre11/Rad50/Nbs1 (MRN) complex.
Application : Functional Assay
Inagaki H et al. Nat Commun 2013; 4:1592 Two sequential cleavage reactions on cruciform DNA structures cause palindrome-mediated chromosomal translocations.
Stewart R et al. Hum Mol Genet 2013; 22 (12):2495-509 A patient-derived olfactory stem cell disease model for ataxia-telangiectasia.
Application : Functional Assay
Sohn SY et al. J Virol 2012; 86 (18):9656-65 Adenovirus regulates sumoylation of Mre11-Rad50-Nbs1 components through a paralog-specific mechanism.
Andrin C et al. Nucleus 2012; 3 (4):384-95 A requirement for polymerized actin in DNA double-strand break repair.
Harding SM et al. Cell Cycle 2012; 11 (7):1432-44 Discordance between phosphorylation and recruitment of 53BP1 in response to DNA double-strand breaks.
Tomimatsu N et al. DNA Repair (Amst) 2012; 11 (4):441-8 Exo1 plays a major role in DNA end resection in humans and influences double-strand break repair and damage signaling decisions.
Karen KA et al. J Virol 2011; 85 (9):4135-42 Adenovirus core protein VII protects the viral genome from a DNA damage response at early times after infection.
Application : ICC/IF
Reactivity : Human
Anderson VE et al. Cancer Res 2011; 71 (2):463-72 CCT241533 is a potent and selective inhibitor of CHK2 that potentiates the cytotoxicity of PARP inhibitors.
Application : WB
Reactivity : Human
Matsumoto Y et al. DNA Repair (Amst) 2011; 10 (3):314-21 Two unrelated patients with MRE11A mutations and Nijmegen breakage syndrome-like severe microcephaly.
Application : WB
Reactivity : Human
Luo Y et al. J Virol 2011; 85 (1):133-45 Bocavirus infection induces a DNA damage response that facilitates viral DNA replication and mediates cell death.
Application : ICC/IF
Reactivity : Human
Masuda Y et al. Biochem Biophys Res Commun 2011; 415 (2):305-12 Novel regulatory role for Kaposi's sarcoma-associated herpesvirus-encoded vFLIP in chemosensitization to bleomycin.
Application : WB
Reactivity : Human
Yanagihara H et al. Mol Cell 2011; 43 (5):788-97 NBS1 recruits RAD18 via a RAD6-like domain and regulates Pol η-dependent translesion DNA synthesis.
Application : WB
Reactivity : Human
Della-Maria J et al. J Biol Chem 2011; 286 (39):33845-53 Human Mre11/human Rad50/Nbs1 and DNA ligase IIIalpha/XRCC1 protein complexes act together in an alternative nonhomologous end joining pathway.
Gatei M et al. J Biol Chem 2011; 286 (36):31542-56 ATM protein-dependent phosphorylation of Rad50 protein regulates DNA repair and cell cycle control.
Baydoun HH et al. Blood 2011; 117 (22):5897-906 Human T-lymphotropic type 1 virus p30 inhibits homologous recombination and favors unfaithful DNA repair.
Application : ICC/IF
Reactivity : Human
Forrester NA et al. J Virol 2011; 85 (5):110244 Serotype-specific inactivation of the cellular DNA damage response during adenovirus infection.
Application : ICC/IF
Reactivity : Human
Kozlov SV et al. J Biol Chem 2011; 286 (11):9107-19 Autophosphorylation and ATM activation: additional sites add to the complexity.
Orazio NI et al. J Virol 2011; 85 (4):1887-92 The adenovirus E1b55K/E4orf6 complex induces degradation of the Bloom helicase during infection.
Application : WB
Reactivity : Human
Adeyemi RO et al. PLoS Pathog 2010; 6 (10):e1001141 Parvovirus minute virus of mice induces a DNA damage response that facilitates viral replication.
Application : WB
Reactivity : Human
Balasubramanian N et al. J Virol 2010; 84 (24):12504-14 Physical interaction between the herpes simplex virus type 1 exonuclease, UL12, and the DNA double-strand break-sensing MRN complex.
Application : WB
Cha H et al. Cancer Res 2010; 70 (10):4112-22 Wip1 directly dephosphorylates gamma-H2AX and attenuates the DNA damage response.
Guo Z et al. Cell Cycle 2010; 9 (24):1061336 ATM activation in the presence of oxidative stress.
Application : WB
Offer SM et al. PLoS One 2010; 5 (8):e12260 Unique DNA repair gene variations and potential associations with the primary antibody deficiency syndromes IgAD and CVID.
Application : WB
Reactivity : Human
Chailleux C et al. Biochem J 2010; 426 (3):365-71 Physical interaction between the histone acetyl transferase Tip60 and the DNA double-strand breaks sensor MRN complex.
Application : WB
Reactivity : Human
Dellaire G et al. Cell Cycle 2009; 8 (22):3750-69 high resolution imaging of changes in the structure and spatial organization of chromatin, gamma-h2a.x and the mrn complex within *-induced dna repair foci.
Application : WB
Reactivity : Human
Moody CA et al. PLoS Pathog 2009; 5 (10):e1000605 Human papillomaviruses activate the ATM DNA damage pathway for viral genome amplification upon differentiation.
Application : WB
Reactivity : Human
Turaga RV et al. Cell Cycle 2009; 8 (13):2080-92 The Werner syndrome protein affects the expression of genes involved in adipogenesis and inflammation in addition to cell cycle and DNA damage responses.
Application : WB
Reactivity : Human
Waltes R et al. Am J Hum Genet 2009; 84 (5):605-16 Human RAD50 deficiency in a Nijmegen breakage syndrome-like disorder.
Application : ICC/IF
Reactivity : Human
Schwartz RA et al. J Virol 2009; 83 (12):6269-78 Adeno-associated virus replication induces a DNA damage response coordinated by DNA-dependent protein kinase.
Application : WB
Reactivity : Human
Zheng L et al. Nucleic Acids Res 2009; 37 (8):2645-57 MRE11 complex links RECQ5 helicase to sites of DNA damage.
Application : WB
Reactivity : Human
Karen KA et al. J Virol 2009; 83 (9):4565-73 Temporal regulation of the Mre11-Rad50-Nbs1 complex during adenovirus infection.
Application : WB
Reactivity : Human
Moschos SJ et al. Cancer Biol Ther 2009; 8 (19):1815-25 Suppressing the high-level expression and function of ATM in advanced-stage melanomas does not sensitize the cells to ionizing radiation.
Lins S et al. Gene 2009; 447 (1):43441 Clinical variability and expression of the NBN c.657del5 allele in Nijmegen Breakage Syndrome.
Application : WB
Reactivity : Human
Collaco RF et al. Virology 2009; 392 (1):24-33 Adeno-associated virus and adenovirus coinfection induces a cellular DNA damage and repair response via redundant phosphatidylinositol 3-like kinase pathways.
Application : ICC/IF
Reactivity : Human
Roques C et al. EMBO J 2009; 28 (16):2400-13 MRE11-RAD50-NBS1 is a critical regulator of FANCD2 stability and function during DNA double-strand break repair.
Application : WB
Reactivity : Human
Vago R et al. Cell Cycle 2009; 8 (16):255853 DNA ligase I and Nbs1 proteins associate in a complex and colocalize at replication factories.
Application : WB
Reactivity : Human
Carlessi L et al. Cell Death Differ 2009; 16 (6):795-806 DNA-damage response, survival and differentiation in vitro of a human neural stem cell line in relation to ATM expression.
Application : WB
Reactivity : Human
Zou Y et al. Mol Cell Biol 2009; 29 (9):179152 Altered states of telomere deprotection and the two-stage mechanism of replicative aging.
Application : ICC/IF
Carson CT et al. EMBO J 2009; 28 (6):652-62 Mislocalization of the MRN complex prevents ATR signaling during adenovirus infection.
Application : ICC/IF
Reactivity : Human
Crosby ME et al. Cancer Res 2009; 69 (3):1221-9 MicroRNA regulation of DNA repair gene expression in hypoxic stress.
Application : WB
Reactivity : Human
Lakdawala SS et al. J Virol 2008; 82 (17):8362-72 Differential requirements of the C terminus of Nbs1 in suppressing adenovirus DNA replication and promoting concatemer formation.
Application : ICC/IF
Reactivity : Human
Cervelli T et al. J Cell Sci 2008; 121 (Pt 3):349-57 Processing of recombinant AAV genomes occurs in specific nuclear structures that overlap with foci of DNA-damage-response proteins.
Application : ICC/IF
Reactivity : Human
Gregory DA et al. Virology 2008; 373 (1):124-36 Characterization of mre11 loss following HSV-1 infection.
Application : ICC/IF
Reactivity : Human
Haince JF et al. J Biol Chem 2008; 283 (2):1197-208 PARP1-dependent kinetics of recruitment of MRE11 and NBS1 proteins to multiple DNA damage sites.
Palii SS et al. Mol Cell Biol 2008; 28 (2):752-71 DNA methylation inhibitor 5-Aza-2'-deoxycytidine induces reversible genome-wide DNA damage that is distinctly influenced by DNA methyltransferases 1 and 3B.
Application : WB
Reactivity : Human
Dery U et al. Mol Cell Biol 2008; 28 (9):3058-69 A glycine-arginine domain in control of the human MRE11 DNA repair protein.
Schwartz RA et al. J Virol 2007; 81 (23):12936-45 The Mre11/Rad50/Nbs1 complex limits adeno-associated virus transduction and replication.
Application : ICC/IF
Reactivity : Human
Murakawa Y et al. Cancer Res 2007; 67 (18):8536-43 Inhibitors of the proteasome suppress homologous DNA recombination in mammalian cells.
Application : ICC/IF
Reactivity : Human
Woo JL et al. J Virol 2007; 81 (2):575-87 Adenovirus ubiquitin-protein ligase stimulates viral late mRNA nuclear export.
Application : WB
Reactivity : Human
Kruger L et al. Carcinogenesis 2007; 28 (1):107-11 Cancer incidence in Nijmegen breakage syndrome is modulated by the amount of a variant NBS protein.
Application : WB
Reactivity : Human
Bentle MS et al. Cancer Res 2007; 67 (14):6936-45 Nonhomologous end joining is essential for cellular resistance to the novel antitumor agent, beta-lapachone.
Application : ICC/IF
Reactivity : Human
Xu M et al. Int J Hyperthermia 2007; 23 (4):343-51 The effects of 41 degrees C hyperthermia on the DNA repair protein, MRE11, correlate with radiosensitization in four human tumor cell lines.
Cho YW et al. J Biol Chem 2007; 282 (28):20395-406 PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 methyltransferase complex.
Application : WB
Reactivity : Human
Fleisig HB et al. Oncogene 2007; 26 (33):4797-805 Adenoviral E1B55K oncoprotein sequesters candidate leukemia suppressor sequence-specific single-stranded DNA-binding protein 2 into aggresomes.
Barcaroli D et al. Proc Natl Acad Sci U S A 2006; 103 (40):14802-7 FLASH is an essential component of Cajal bodies.
Application : ICC/IF
Reactivity : Human
Barcaroli D et al. Proc Natl Acad Sci U S A 2006; 103 (40):14808-12 FLASH is required for histone transcription and S-phase progression.
Application : ICC/IF
Reactivity : Human
Dote H et al. Cancer Res 2006; 66 (18):9211-20 Inhibition of hsp90 compromises the DNA damage response to radiation.
Application : ICC/IF
Reactivity : Human
Nakanishi K et al. Arch Pathol Lab Med 2006; 130 (9):1330-4 Mre11 expression in atypical adenomatous hyperplasia and adenocarcinoma of the lung.
Application : IHC-P
Reactivity : Human
Lovejoy CA et al. Mol Cell Biol 2006; 26 (21):7977-90 DDB1 maintains genome integrity through regulation of Cdt1.
Application : ICC/IF
Reactivity : Human
Takemura H et al. J Biol Chem 2006; 281 (41):30814-23 Defective Mre11-dependent activation of Chk2 by ataxia telangiectasia mutated in colorectal carcinoma cells in response to replication-dependent DNA double strand breaks.
Application : WB
Reactivity : Human
Kozlov SV et al. EMBO J 2006; 25 (15):3504-14 Involvement of novel autophosphorylation sites in ATM activation.
Application : WB
Reactivity : Human
Bekker-Jensen S et al. J Cell Biol 2006; 173 (2):195-206 Spatial organization of the mammalian genome surveillance machinery in response to DNA strand breaks.
Application : ICC/IF
Reactivity : Human
Gueven N et al. Cancer Res 2006; 66 (6):368135 Defective p53 response and apoptosis associated with an ataxia-telangiectasia-like phenotype.
Application : WB
Reactivity : Human
Varon R et al. Hum Mol Genet 2006; 15 (5):679-89 Mild Nijmegen breakage syndrome phenotype due to alternative splicing.
Application : WB
Reactivity : Human
Chai W et al. EMBO Rep 2006; 7 (2):225-30 The involvement of the Mre11/Rad50/Nbs1 complex in the generation of G-overhangs at human telomeres.
Application : WB
Reactivity : Human
Vo AT et al. EMBO Rep 2005; 6 (5):438-44 hMRE11 deficiency leads to microsatellite instability and defective DNA mismatch repair.
Application : WB
Reactivity : Human
Lilley CE et al. Proc Natl Acad Sci U S A 2005; 102 (16):1440762 DNA repair proteins affect the lifecycle of herpes simplex virus 1.
Application : ICC/IF
Reactivity : Human
Hart LS et al. J Biol Chem 2005; 280 (2):1474-81 The adenovirus E4orf6 protein inhibits DNA double strand break repair and radiosensitizes human tumor cells in an E1B-55K-independent manner.
Application : WB
Reactivity : Human
Al Rashid ST et al. Cancer Res 2005; 65 (23):10810-21 Evidence for the direct binding of phosphorylated p53 to sites of DNA breaks in vivo.
Application : ICC/IF
Reactivity : Human
Kim JS et al. J Cell Biol 2005; 170 (3):341-7 Independent and sequential recruitment of NHEJ and HR factors to DNA damage sites in mammalian cells.
Application : ICC/IF
Reactivity : Human
van Veelen LR et al. Mutat Res 2005; 574 (43102):22-33 Analysis of ionizing radiation-induced foci of DNA damage repair proteins.
Application : ICC/IF
Fan R et al. Cancer Res 2004; 64 (23):8526-33 Defective DNA strand break repair after DNA damage in prostate cancer cells: implications for genetic instability and prostate cancer progression.
Application : WB
Reactivity : Human
Zhang X et al. Mol Cell Biol 2004; 24 (20):9207-20 Artemis is a phosphorylation target of ATM and ATR and is involved in the G2/M DNA damage checkpoint response.
Application : WB
Reactivity : Human
Pichierri P et al. EMBO J 2004; 23 (15):3154-63 BLM and the FANC proteins collaborate in a common pathway in response to stalled replication forks.
Application : ICC/IF
Reactivity : Human
Robison JG et al. J Biol Chem 2004; 279 (33):34802-10 Replication protein A and the Mre11.Rad50.Nbs1 complex co-localize and interact at sites of stalled replication forks.
Application : ICC/IF
Reactivity : Human
Wilkinson DE et al. J Virol 2004; 78 (9):4783-96 Recruitment of cellular recombination and repair proteins to sites of herpes simplex virus type 1 DNA replication is dependent on the composition of viral proteins within prereplicative sites and correlates with the induction of the DNA damage response.
Application : WB
Dork T et al. Am J Med Genet A 2004; 126A (3):272-7 Slow progression of ataxia-telangiectasia with double missense and in frame splice mutations.
Application : WB
Giannini G et al. Oncogene 2004; 23 (15):270463 Mutations of an intronic repeat induce impaired MRE11 expression in primary human cancer with microsatellite instability.
Application : ICC/IF
Reactivity : Human
Horejsi Z et al. Oncogene 2004; 23 (17):446509 Distinct functional domains of Nbs1 modulate the timing and magnitude of ATM activation after low doses of ionizing radiation.
Application : ICC/IF
Stewart GS et al. Nature 2003; 421 (6926):961-6 MDC1 is a mediator of the mammalian DNA damage checkpoint.
Lukas C et al. Nat Cell Biol 2003; 5 (3):255-60 Distinct spatiotemporal dynamics of mammalian checkpoint regulators induced by DNA damage.
Xu X et al. J Biol Chem 2003; 278 (10):8795-803 NFBD1/KIAA0170 is a chromatin-associated protein involved in DNA damage signaling pathways.
Application : ICC/IF
Reactivity : Human
Carson CT et al. EMBO J 2003; 22 (24):6610-20 The Mre11 complex is required for ATM activation and the G2/M checkpoint.
Application : WB
Reactivity : Human
Uziel T et al. EMBO J 2003; 22 (20):5612-21 Requirement of the MRN complex for ATM activation by DNA damage.
Application : ICC/IF
Reactivity : Human
Xu X et al. FASEB J 2003; 17 (13):1842-8 NFBD1/MDC1 regulates ionizing radiation-induced focus formation by DNA checkpoint signaling and repair factors.
Application : ICC/IF
Reactivity : Human
Gottwein E et al. J Virol 2003; 77 (17):9474-85 The Mason-Pfizer monkey virus PPPY and PSAP motifs both contribute to virus release.
Lee JH et al. Mol Cancer Res 2003; 1 (9):674-81 Distinct functions of Nijmegen breakage syndrome in ataxia telangiectasia mutated-dependent responses to DNA damage.
Application : ICC/IF
Reactivity : Human
Celeste A et al. Nat Cell Biol 2003; 5 (7):675-9 Histone H2AX phosphorylation is dispensable for the initial recognition of DNA breaks.
Her C et al. DNA Repair (Amst) 2002; 1 (9):719-29 Evidence for a direct association of hMRE11 with the human mismatch repair protein hMLH1.
Nakanishi K et al. Nat Cell Biol 2002; 4 (12):913-20 Interaction of FANCD2 and NBS1 in the DNA damage response.
Richie CT et al. Mol Cell Biol 2002; 22 (24):8635-47 hSnm1 colocalizes and physically associates with 53BP1 before and after DNA damage.
Application : ICC/IF
Reactivity : Human
Pichierri P et al. Hum Mol Genet 2002; 11 (21):2531-46 DNA cross-link-dependent RAD50/MRE11/NBS1 subnuclear assembly requires the Fanconi anemia C protein.
Application : WB
Kim JS et al. J Biol Chem 2002; 277 (47):45149-53 Specific recruitment of human cohesin to laser-induced DNA damage.
Application : ICC/IF
Reactivity : Human
Xu M et al. Int J Hyperthermia 2002; 18 (5):385-403 Radiosensitization of heat resistant human tumour cells by 1 hour at 41.1 degrees C and its effect on DNA repair.
Stracker TH et al. Nature 2002; 418 (6895):348-52 Adenovirus oncoproteins inactivate the Mre11-Rad50-NBS1 DNA repair complex.
Application : ICC/IF
Reactivity : Human
Limoli CL et al. Proc Natl Acad Sci U S A 2002; 99 (1):233-8 UV-induced replication arrest in the xeroderma pigmentosum variant leads to DNA double-strand breaks, gamma -H2AX formation, and Mre11 relocalization.
Application : ICC/IF
Reactivity : Human
Gatei M et al. Nat Genet 2000; 25 (1):115-9 ATM-dependent phosphorylation of nibrin in response to radiation exposure.
Wang Y et al. Genes Dev 2000; 14 (8):927-39 BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures.
Application : ICC/IF
Reactivity : Human
Harfst E et al. Mol Immunol 2000; 37 (15):915-29 Normal V(D)J recombination in cells from patients with Nijmegen breakage syndrome.
Application : WB
Lombard DB et al. Cancer Res 2000; 60 (9):157511 Nijmegen breakage syndrome disease protein and MRE11 at PML nuclear bodies and meiotic telomeres.
Application : ICC/IF
Reactivity : Human
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