For WB: Use at a dilution of 1:500-1:3000. For ICC/IF: Use at a dilution of 1:100 - 1:200. For IHC-P: Use at a dilution of 1:200. Incubation with the primary antibody was preceded by microwave treatment of the tissue section for antigen retrieval. For IP: Use at a dilution of 1:100. Predicted molecular weight: 79 kDa. Optimal dilutions/concentrations should be determined by the researcher.
HeLa , Jurkat , Raji , NCI-H929 , 293T , human Mre11-transfected 293T , PC-12
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1.89mg/ml(Please refer to the vial label for the specific concentration.)
Amino acids 182-582 of Mre11 expressed in E. coli.
Protein G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
MRE11, MRE11B, P49959, 600814, ATLD, 4361, MRE11A, HNGS1
Mre11 is an 80 kDa protein that acts as a Mn+2 dependent double stranded DNA 3' to 5' exonuclease and a single stranded DNA endonuclease. It is part of a larger complex with Rad50, p95 (Nibrin) and possibly other associated proteins involved in the nucleolytic end-processing of double strand breaks and telomere maintenance.