PRODUCT
Summary
MRE11 antibody recognizes MRE11 protein, an enzyme with a predicted molecular weight of ~81 kDa. MRE11 forms the core of the MRN (MRE11-RAD50-NBS1) complex, one of the first components of the DNA damage response (DDR) to DNA double-strand breaks where it recruits other signaling factors required for repair. MRE11 has both exonuclease and endonuclease activities, and is responsible for the DNA binding of the complex.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HeLa , Jurkat , Raji , NCI-H929 , 293T , human Mre11-transfected 293T , PC-12
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.45 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Amino acids 182-582 of Mre11 expressed in E. coli.
Purification
Protein G purified
Conjugation
Unconjugated
RRID
AB_372398
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
MRE11 homolog, double strand break repair nuclease , ATLD , HNGS1 , MRE11A , MRE11B
Cellular Localization
Nucleus
Background
This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX70212 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (GTX70212) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX70212 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Mre11 antibody [12D7] (GTX70212) diluted at 1:1000 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX70212 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Mre11 antibody [12D7] (GTX70212) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX70212 IHC-P Image
Mre11 antibody [12D7] detects Mre11 protein at nucleus by immunohistochemical analysis (Autostainer Formulated).Sample: Paraffin-embedded human colon cancer.Mre11 stained by Mre11 antibody [12D7] (GTX70212) diluted at 1:300.Antigen Retrieval: EDTA buffer, 20 min
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GTX70212 WB Image
Mre11 antibody [12D7] detects Mre11 protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg whole cell extract of human Mre11-transfected 293T cells 7.5% SDS-PAGE Mre11 antibody [12D7] (GTX70212) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX70212 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (GTX70212) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX70212 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (GTX70212) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX70212 ICC/IF Image
Mre11 antibody [12D7] detects Mre11 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Mre11 stained by Mre11 antibody [12D7] (GTX70212) diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar= 10 μm.
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REFERENCE
REVIEW
Mre11 antibody [12D7]
Cat. No. GTX70212
Rating
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( Average 5 based on 14 users reviews)
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Application
Western Blot(WB)
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( Average 5 based on 7 users reviews)
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Immunocytochemistry/ Immunofluorescence(ICC/IF)
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( Average 5 based on 6 users reviews)
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IHC (Paraffin sections)(IHC-P)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 18-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
HaCaT cells
Amount used :
4 μg
Blocking :
Skim milk, 25°C, 1Hr
Primary Antibody :
1:3000, 4°C, 11Hr
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Open Biol in 2015.
PMID: 25924630
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2015.
PMID: 25826455
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Nat Commun in 2017.
PMID: 29042561
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal BMC Cancer in 2014.
PMID: 24928521
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Nat Commun in 2017.
PMID: 29042561
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
ICC/IF
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Open Biol in 2015.
PMID: 25924630
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
ICC/IF
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal J Virol in 2017.
PMID: 28855246
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
ICC/IF
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Viruses in 2015.
PMID: 26057167
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
ICC/IF
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS Pathog in 2010.
PMID: 20949077
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal BMC Biol in 2015.
PMID: 25857663
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
ICC/IF
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Nucleic Acids Res in 2016.
PMID: 26446986
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
ICC/IF
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Nucleic Acids Res in 2016.
PMID: 26446986
Date :
Anonymous submitted on 21-Oct-2020
Application Tested :
IHC-P
Sample Species :
Hu
Sample :
colon cancer tissue
Fixation :
EDTA buffer, 0.3°C
Primary Antibody :
1:300, 4°C, 16Hr