GeneTex
  • Country / Location Selection

United States (US)

Mre11 antibody [12D7]

Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Anti-Mre11 antibody [12D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70212
Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Anti-Mre11 antibody [12D7] used in IHC (Paraffin sections) (IHC-P). GTX70212

Cat No. GTX70212

Host

Mouse

Clonality

Monoclonal

Clone Name

12D7

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IP, ELISA, Functional Assay, PLA

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)
PRODUCT

Summary

MRE11 antibody recognizes MRE11 protein, an enzyme with a predicted molecular weight of ~81 kDa. MRE11 forms the core of the MRN (MRE11-RAD50-NBS1) complex, one of the first components of the DNA damage response (DDR) to DNA double-strand breaks where it recruits other signaling factors required for repair. MRE11 has both exonuclease and endonuclease activities, and is responsible for the DNA binding of the complex.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP Assay dependent
ELISA Assay dependent
Functional Assay Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

HeLa , Jurkat , Raji , NCI-H929 , 293T , human Mre11-transfected 293T , PC-12

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 182-582 of Mre11 expressed in E. coli.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_372398

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MRE11 homolog, double strand break repair nuclease , ATLD , HNGS1 , MRE11A , MRE11B

Cellular Localization

Nucleus

Background

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212

GTX70212 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (GTX70212) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212

GTX70212 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Mre11 antibody [12D7] (GTX70212) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212

GTX70212 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (GTX70212) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212

GTX70212 WB Image

Mre11 antibody [12D7] detects Mre11 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of human Mre11-transfected 293T cells
7.5% SDS-PAGE
Mre11 antibody [12D7] (GTX70212) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Mre11 antibody [12D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70212

GTX70212 ICC/IF Image

Mre11 antibody [12D7] detects Mre11 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Mre11 stained by Mre11 antibody [12D7] (GTX70212) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212

GTX70212 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (GTX70212) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212

GTX70212 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (GTX70212) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Mre11 antibody [12D7] used in IHC (Paraffin sections) (IHC-P). GTX70212

GTX70212 IHC-P Image

Mre11 antibody [12D7] detects Mre11 protein at nucleus by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human colon cancer.
Mre11 stained by Mre11 antibody [12D7] (GTX70212) diluted at 1:300.
Antigen Retrieval: EDTA buffer, 20 min

REFERENCE

REVIEW

Mre11 antibody [12D7] Cat No. GTX70212

Rating ( Average 5 based on 14 users reviews)
Application
Western Blot(WB) ( Average 5 based on 8 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 5 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : HaCaT cells
Amount used : 4 μg
Blocking : Skim milk, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, 11Hr
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Open Biol in 2015.PMID: 25924630
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 25826455
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017.PMID: 29042561
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal BMC Cancer in 2014.PMID: 24928521
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017.PMID: 29042561
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Open Biol in 2015.PMID: 25924630
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Virol in 2017.PMID: 28855246
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Viruses in 2015.PMID: 26057167
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2010.PMID: 20949077
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal BMC Biol in 2015.PMID: 25857663
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nucleic Acids Res in 2016.PMID: 26446986
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nucleic Acids Res in 2016.PMID: 26446986
Date : Anonymous submitted on 21-Oct-2020
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : colon cancer tissue
Fixation : EDTA buffer, 0.3°C
Primary Antibody : 1:300, 4°C, 16Hr
Application Reference
SDS
PBS.pdf