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Mre11 antibody [12D7]

Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212
Anti-Mre11 antibody [12D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70212

Cat No. GTX70212

Host Mouse
Clonality Monoclonal
Clone Name 12D7
Isotype IgG1
Application WB, ICC/IF, IHC-P, IP, ELISA, Functional Assay, PLA
Reactivity Human, Mouse, Rat
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP Assay dependent
ELISA Assay dependent
Functional Assay Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

HeLa , Jurkat , Raji , NCI-H929 , 293T , human Mre11-transfected 293T , PC-12
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 182-582 of Mre11 expressed in E. coli.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mre11 Homolog, Double Strand Break Repair Nuclease,Atld,Hngs1,Mre11A,Mre11B,Mre11

Cellular Localization

Nuclear

Background

Mre11 is an 80 kDa protein that acts as a Mn+2 dependent double stranded DNA 3' to 5' exonuclease and a single stranded DNA endonuclease. It is part of a larger complex with Rad50, p95 (Nibrin) and possibly other associated proteins involved in the nucleolytic end-processing of double strand breaks and telomere maintenance.

Database

Research Area

DATA IMAGES
Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212

GTX70212 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (GTX70212) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212

GTX70212 WB Image

Mre11 antibody [12D7] detects Mre11 protein by western blot analysis.
A. 30 µg Jurkat whole cell extract
B. 30 µg Raji whole cell extract
C. 30 µg NCI-H929 whole cell extract
7.5% SDS-PAGE
Mre11 antibody [12D7] (GTX70212) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212

GTX70212 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (GTX70212) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212

GTX70212 WB Image

Mre11 antibody [12D7] detects Mre11 protein by western blot analysis.
A. 30 µg 293T whole cell extract
B. 30 µg whole cell extract of human Mre11-transfected 293T cells
7.5% SDS-PAGE
Mre11 antibody [12D7] (GTX70212) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Mre11 antibody [12D7] used in Western Blot (WB). GTX70212

GTX70212 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (GTX70212) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Mre11 antibody [12D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70212

GTX70212 ICC/IF Image

Mre11 antibody [12D7] detects Mre11 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Mre11 stained by Mre11 antibody [12D7] (GTX70212) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

REFERENCE
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Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 319