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Mre11 antibody [C1C3-2]

KO/KD Comparison Overexpression
Anti-Mre11 antibody [C1C3-2] used in Western Blot (WB). GTX111814
Anti-Mre11 antibody [C1C3-2] used in Western Blot (WB). GTX111814
Anti-Mre11 antibody [C1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111814
Anti-Mre11 antibody [C1C3-2] used in Western Blot (WB). GTX111814
Anti-Mre11 antibody [C1C3-2] used in Immunoprecipitation (IP). GTX111814

Cat. No. GTX111814

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Mre11 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

HeLa , MRE11A-transfected 293T

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Mre11. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167298

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MRE11 homolog, double strand break repair nuclease , ATLD , HNGS1 , MRE11A , MRE11B

Cellular Localization

Nucleus

Background

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Mre11 antibody [C1C3-2] used in Western Blot (WB). GTX111814

GTX111814 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [C1C3-2] (GTX111814) diluted at 1:1000.

Anti-Mre11 antibody [C1C3-2] used in Western Blot (WB). GTX111814

GTX111814 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Mre11 antibody [C1C3-2] (GTX111814) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Mre11 antibody [C1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111814

GTX111814 ICC/IF Image

Mre11 antibody [C1C3-2] detects Mre11 protein at nucleus by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Mre11 protein stained by Mre11 antibody [C1C3-2] (GTX111814) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Mre11 antibody [C1C3-2] used in Western Blot (WB). GTX111814

GTX111814 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [C1C3-2] (GTX111814) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Mre11 antibody [C1C3-2] used in Immunoprecipitation (IP). GTX111814

GTX111814 IP Image

Immunoprecipitation of Mre11 protein from HeLa whole cell extracts using 5 μg of Mre11 antibody [C1C3-2] (GTX111814).
Western blot analysis was performed using Mre11 antibody [C1C3-2] (GTX111814).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169