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Mre11 antibody

KO/KD Orthogonal Overexpression
Anti-Mre11 antibody used in Western Blot (WB). GTX118741
Anti-Mre11 antibody used in Western Blot (WB). GTX118741
Anti-Mre11 antibody used in IHC (Paraffin sections) (IHC-P). GTX118741
Anti-Mre11 antibody used in Western Blot (WB). GTX118741
Anti-Mre11 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118741
Anti-Mre11 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118741
Anti-Mre11 antibody used in Immunoprecipitation (IP). GTX118741
Anti-Mre11 antibody used in Western Blot (WB). GTX118741
Anti-Mre11 antibody used in Western Blot (WB). GTX118741
Anti-Mre11 antibody used in Western Blot (WB). GTX118741
Anti-Mre11 antibody used in Western Blot (WB). GTX118741

Cat. No. GTX118741

Reference 7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Mre11 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

A431 , C8D30 , NIH-3T3 , PC-12 , HeLa , MRE11A-transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Mre11. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624939

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MRE11 homolog, double strand break repair nuclease , ATLD , HNGS1 , MRE11A , MRE11B

Cellular Localization

Nucleus

Background

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Mre11 antibody used in Western Blot (WB). GTX118741

GTX118741 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody (GTX118741) diluted at 1:2000.

Anti-Mre11 antibody used in Western Blot (WB). GTX118741

GTX118741 WB Image

Mre11 antibody detects MRE11A protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
7.5 % SDS-PAGE
Mre11 antibody (GTX118741) dilution: 1:1000

Anti-Mre11 antibody used in IHC (Paraffin sections) (IHC-P). GTX118741

GTX118741 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Mre11(GTX118741) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Mre11 antibody used in Western Blot (WB). GTX118741

GTX118741 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody (GTX118741) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Mre11 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118741

GTX118741 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Mre11(GTX118741) antibody at 1:200 dilution.

Anti-Mre11 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118741

GTX118741 ICC/IF Image

Mre11 antibody detects Mre11 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Mre11 stained by Mre11 antibody (GTX118741) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Scale bar= 10μm.

Anti-Mre11 antibody used in Immunoprecipitation (IP). GTX118741

GTX118741 IP Image

Immunoprecipitation of Mre11 protein from HeLa whole cell extracts using 5 μg of Mre11 antibody (GTX118741).
Western blot analysis was performed using Mre11 antibody (GTX118741).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Mre11 antibody used in Western Blot (WB). GTX118741

GTX118741 WB Image

Mre11 antibody detects MRE11A protein by Western blot analysis.
A. 30 μg C8D30 whole cell lysate/extract
B. 30 μg NIH-3T3 whole cell lysate/extract
7.5 % SDS-PAGE
Mre11 antibody (GTX118741) dilution: 1:1000

Anti-Mre11 antibody used in Western Blot (WB). GTX118741

GTX118741 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody (GTX118741) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Mre11 antibody used in Western Blot (WB). GTX118741

GTX118741 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2020.PMID: 31980707

Anti-Mre11 antibody used in Western Blot (WB). GTX118741

GTX118741 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2020.PMID: 31980707

REFERENCE

Application Reference

Cheng SM et al. Pharmacol Res 2021; 166 : 105474 YM155 and BIRC5 downregulation induce genomic instability via autophagy-mediated ROS production and inhibition in DNA repair.
Application : WB
Reactivity : Human
Honda T et al. Sci Rep 2020; 10 (1) : 1140 PDZRN3 protects against apoptosis in myoblasts by maintaining cyclin A2 expression.
Application : WB
Reactivity : Mouse
Hagkarim NC et al. J Virol 2018; (Epub) Degradation of a novel DNA damage response protein, tankyrase 1 binding protein 1 (Tab182), following adenovirus infection.
Application : WB
Reactivity : Human
Eccles LJ et al. DNA Repair (Amst) 2018; 64 : 43109 Inhibition of non-homologous end joining in Fanconi Anemia cells results in rescue of survival after interstrand crosslinks but sensitization to replication associated double-strand breaks.
Application : ICC/IF
Reactivity : Human
Pantelidou C et al. Oncotarget 2016; (Epub) The E1B19K-deleted oncolytic adenovirus mutant AdΔ19K sensitizes pancreatic cancer cells to drug-induced DNA-damage by down-regulating Claspin and Mre11.
Application : ICC/IF
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169