GeneTex

  • Country / Location Selection

United States (US)

Mre11 antibody

Anti-Mre11 antibody used in ChIP assay (ChIP assay). GTX12159

Cat. No. GTX12159

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA, ChIP assay

Reactivity

 Saccharomyces cerevisiae
Package
100 μg ($589)
See all Mre11 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ELISA 1:10000-1:50000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Saccharomyces cerevisiae

Immunogen

Synthetic peptide corresponding to an internal region near amino acids 575-600 of Saccharomyces cerevisiae (baker's yeast) Mre11 protein.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

RRID

AB_625966

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MRE11 homolog, double strand break repair nuclease , ATLD , HNGS1 , MRE11A , MRE11B

Cellular Localization

Nucleus

Background

Mre11 is an 80 kDa protein that acts as a Mn2+ dependent double stranded DNA 3' to 5' exonuclease and a single stranded DNA endonuclease. It is part of a larger complex involved in the nucleolytic end-processing of double strand breaks and telomere maintenance.

Database

Research Area

DATA IMAGES

Anti-Mre11 antibody used in ChIP assay (ChIP assay). GTX12159

GTX12159 ChIP assay Image

Chromatin Immunoprecipitation (ChIP) using GeneTex Affinity Purified Mre11 (S. cerevisiae) antibody (GTX12159). A yeast strain containing the HO endonuclease gene controlled by a galactose-inducible promoter (uninduced 0 m lanes) was shifted into galactose containing medium (induced 60 m lanes). After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of sheared chromatin. Chromatin was immunoprecipitated with the antibody at the stated dilutions. Immunocomplexes were captured using polyacrylamide bead linked secondary antibodies. The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower arrow) and 67 kb from the break (upper band, control locus). PCR products were displayed on a polyacrylamide gel, stained with SyBR GreenR (Invitrogen), and detected using a Fuji scanning fluorometer.

REFERENCE

Application Reference

Hamilton NK et al. PLoS One 2010; 5 (10) : e15387 MRE11 function in response to topoisomerase poisons is independent of its function in double-strand break repair in Saccharomyces cerevisiae.
Application : WB
Submit a Reference

REVIEW

There are currently no reviews for Mre11 antibody (GTX12159). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 589