GeneTex
United States (US)

Mre11 antibody

GTX30294_203_ICC-IF_18121410_883.jpg
GTX30294_1387_WB_18121410_328.jpg
GTX30294_1386_WB_18121410_778.jpg
GTX30294_320_IHC-P_18121410_600.jpg

Cat No. GTX30294

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ChIP assay
Reactivity Human, Mouse, Rat, Hamster, Chicken
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000
ICC/IF 1:200
IHC-P 1:10 - 1:500
IHC-Fr Assay dependent
FACS Assay dependent
IP 3 ul
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

81 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Serum, 0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Mre11 protein. [UniProt# P49959]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mre11 Homolog, Double Strand Break Repair Nuclease,Atld,Hngs1,Mre11A,Mre11B,Mre11

Cellular Localization

Nucleus

Background

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30294_203_ICC-IF_18121410_883.jpg

GTX30294 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30294 Mre11 antibody.
Green : primary antibody
Red : Actin
Blue : DAPI
Dilution : 1:500
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100

GTX30294_1387_WB_18121410_328.jpg

GTX30294 WB Image

WB analysis of HeLa cell lysate using GTX30294 Mre11 antibody.

GTX30294_1386_WB_18121410_778.jpg

GTX30294 WB Image

WB analysis of HeLa and MEF cell lysate using GTX30294 Mre11 antibody.

GTX30294_320_IHC-P_18121410_600.jpg

GTX30294 IHC-P Image

IHC-P analysis of human epidermis tissue cross section using GTX30294 Mre11 antibody.

REFERENCE
Package List Price ($)
$ 329