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Mre11 antibody

Anti-Mre11 antibody used in Western Blot (WB). GTX30294
Anti-Mre11 antibody used in IHC (Paraffin sections) (IHC-P). GTX30294
Anti-Mre11 antibody used in Western Blot (WB). GTX30294
Anti-Mre11 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30294

Cat. No. GTX30294

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ChIP assay

Reactivity

 Human, Mouse, Rat, Hamster, Chicken
Package
50 μl ($419)
See all Mre11 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000
ICC/IF 1:200
IHC-P 1:10 - 1:500
IHC-Fr Assay dependent
FACS Assay dependent
IP 3 ul
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Full length human Mre11 protein. [UniProt# P49959]

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_374407

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MRE11 homolog, double strand break repair nuclease , ATLD , HNGS1 , MRE11A , MRE11B

Cellular Localization

Nucleus

Background

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Mre11 antibody used in Western Blot (WB). GTX30294

GTX30294 WB Image

WB analysis of HeLa cell lysate using GTX30294 Mre11 antibody.

Anti-Mre11 antibody used in IHC (Paraffin sections) (IHC-P). GTX30294

GTX30294 IHC-P Image

IHC-P analysis of human epidermis tissue cross section using GTX30294 Mre11 antibody.

Anti-Mre11 antibody used in Western Blot (WB). GTX30294

GTX30294 WB Image

WB analysis of HeLa and MEF cell lysate using GTX30294 Mre11 antibody.

Anti-Mre11 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30294

GTX30294 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30294 Mre11 antibody.
Green : primary antibody
Red : Actin
Blue : DAPI
Dilution : 1:500
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100

REFERENCE

Application Reference

Kim H et al. J Biol Chem 2023; 299 (1) : 102752 Deubiquitinase USP2 stabilizes the MRE11-RAD50-NBS1 complex at DNA double-strand break sites by counteracting the ubiquitination of NBS1.
Application : WB, ICC/IF
Reactivity : Human
Kim H et al. J Biol Chem 2021; 297 (4) : 101148 Stable maintenance of the Mre11-Rad50-Nbs1 complex is sufficient to restore the DNA double-strand break response in cells lacking RecQL4 helicase activity.
Application : WB, ICC/IF
Reactivity : Human
Franchitto A et al. J Cell Biol 2002; 157 (1) : 19-30 Bloom's syndrome protein is required for correct relocalization of RAD50/MRE11/NBS1 complex after replication fork arrest.
Application : ICC/IF
Reactivity : Human

Clone Reference

Siwicki JK et al. Exp Cell Res 2003; 287 (1):178-89 Telomere maintenance and cell cycle regulation in spontaneously immortalized T-cell lines from Nijmegen breakage syndrome patients.
Limoli CL et al. Mutat Res 2002; 510 (43102):121-9 DNA replication arrest in XP variant cells after UV exposure is diverted into an Mre11-dependent recombination pathway by the kinase inhibitor wortmannin.
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REVIEW

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Application Reference
Kim H et al. J Biol Chem 2023; 299 (1):102752 Deubiquitinase USP2 stabilizes the MRE11-RAD50-NBS1 complex at DNA double-strand break sites by counteracting the ubiquitination of NBS1.
Application : WB, ICC/IF
Reactivity : Human
Kim H et al. J Biol Chem 2021; 297 (4):101148 Stable maintenance of the Mre11-Rad50-Nbs1 complex is sufficient to restore the DNA double-strand break response in cells lacking RecQL4 helicase activity.
Application : WB, ICC/IF
Reactivity : Human
Franchitto A et al. J Cell Biol 2002; 157 (1):19-30 Bloom's syndrome protein is required for correct relocalization of RAD50/MRE11/NBS1 complex after replication fork arrest.
Application : ICC/IF
Reactivity : Human
Wang Y et al. Genes Dev 2000; 14 (8):927-39 BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures.
Reactivity : Human
Clone Reference
Siwicki JK et al. Exp Cell Res 2003; 287 (1):178-89 Telomere maintenance and cell cycle regulation in spontaneously immortalized T-cell lines from Nijmegen breakage syndrome patients.
Cleaver JE et al. DNA Repair (Amst) 2002; 1 (1):41-57 Polymerase eta and p53 jointly regulate cell survival, apoptosis and Mre11 recombination during S phase checkpoint arrest after UV irradiation.
Her C et al. DNA Repair (Amst) 2002; 1 (9):719-29 Evidence for a direct association of hMRE11 with the human mismatch repair protein hMLH1.
Limoli CL et al. Mutat Res 2002; 510 (43102):121-9 DNA replication arrest in XP variant cells after UV exposure is diverted into an Mre11-dependent recombination pathway by the kinase inhibitor wortmannin.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 419