WB analysis of recombinant mouse Mre11 protein using GTX48735 Mre11 antibody.
Lane 1 : 0.5 μg
Lane 2 : 0.3 μg
Lane 3 : 0.1 μg
Lane 4 : 0.05 μg
Dilution : 1:1000

Western blot using GeneTex's Affinity Purified anti-Mre11 antibody shows detection of a band ~80 kDa corresponding to mouse Mre11 (arrowhead). Lanes 1-4 contain 0.5 μg, 0.3 μg, 0.1 μg and 0.05 μg of purified mouse Mre11 protein, respectively. After 4-20% SDS-PAGE and transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:1,000 overnight at 4ºC. The membrane was then washed and reacted with a 1:10,000 dilution of IRDye800 conjugated goat anti-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.