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Mtor antibody

Anti-Mtor antibody used in IHC (Paraffin sections) (IHC-P). GTX124771
Anti-Mtor antibody used in Western Blot (WB). GTX124771
Anti-Mtor antibody used in IHC (Whole mount) (IHC-Wm). GTX124771
Anti-Mtor antibody used in Western Blot (WB). GTX124771

Cat. No. GTX124771

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Mtor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm 1:100-1:500
Not tested in other applications.

Predict Reactivity

Human (100%)| Mouse (99%)| Rat (100%)| Zebrafish (90%)| Sheep (98%)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Mtor

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164562

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mechanistic target of rapamycin kinase , frap1 , tor , wu:fc22h08

Database

Research Area

DATA IMAGES

Anti-Mtor antibody used in IHC (Paraffin sections) (IHC-P). GTX124771

GTX124771 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using mtor (GTX124771) antibody at 1:300 dilution.

Anti-Mtor antibody used in Western Blot (WB). GTX124771

GTX124771 WB Image

Various tissue extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Mtor antibody (GTX124771) diluted at 1:1000.

Anti-Mtor antibody used in IHC (Whole mount) (IHC-Wm). GTX124771

GTX124771 IHC-Wm Image

Mtor antibody detects Mtor protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Mtor antibody (GTX124771) dilution: 1:100.
Anti-Mtor antibody used in Western Blot (WB). GTX124771

GTX124771 WB Image

The data was published in the journal Cells in 2019. PMID: 30823450

REFERENCE

Application Reference

Mohapatra S et al. Cell Death Differ 2020; (Epub) Estrogen and estrogen receptors chauffeur the sex-biased autophagic action in liver.
Application : WB
Gu Y et al. Cells 2019; 8 (3) GSK-3β at the Crossroads in Regulating Protein Synthesis and Lipid Deposition in Zebrafish.
Shamay-Ramot A et al. PLoS Genet 2015; 11 (12) : e1005702 Fmrp Interacts with Adar and Regulates RNA Editing, Synaptic Density and Locomotor Activity in Zebrafish.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169