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Mucin 4 X + Mucin 4 Y antibody [HL2026]

Recombinant antibody Overexpression
Anti-Mucin 4 X + Mucin 4 Y antibody [HL2026] used in Western Blot (WB). GTX637927
Anti-Mucin 4 X + Mucin 4 Y antibody [HL2026] used in Western Blot (WB). GTX637927
Anti-Mucin 4 X + Mucin 4 Y antibody [HL2026] used in Western Blot (WB). GTX637927
Anti-Mucin 4 X + Mucin 4 Y antibody [HL2026] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637927

Cat. No. GTX637927

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2026

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Mucin 4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:100000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

232 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Mucin 4 X + Mucin 4 Y.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mucin 4, cell surface associated , ASGP , HSA276359 , MUC-4

Cellular Localization

Membrane,Secreted

Background

The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of this gene have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. This gene contains a region in the coding sequence which has a variable number (>100) of 48 nt tandem repeats. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Mucin 4 X + Mucin 4 Y antibody [HL2026] used in Western Blot (WB). GTX637927

GTX637927 WB Image

Various tissue extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Mucin 4 X + Mucin 4 Y antibody [HL2026] (GTX637927) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Mucin 4 X + Mucin 4 Y antibody [HL2026] used in Western Blot (WB). GTX637927

GTX637927 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Mucin 4 X + Mucin 4 Y antibody [HL2026] (GTX637927) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Mucin 4 X + Mucin 4 Y antibody [HL2026] used in Western Blot (WB). GTX637927

GTX637927 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with Mucin 4 X + Mucin 4 Y antibody [HL2026] (GTX637927) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Mucin 4 X + Mucin 4 Y antibody [HL2026] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637927

GTX637927 ICC/IF Image

Mucin 4 X + Mucin 4 Y antibody [HL2026] detects Mucin 4 X + Mucin 4 Y protein at cell membrane by immunofluorescent analysis.
Sample: NCI-H441 cells were fixed in ice-cold MeOH for 5 min.
Green: Mucin 4 X + Mucin 4 Y stained by Mucin 4 X + Mucin 4 Y antibody [HL2026] (GTX637927) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations