GeneTex
United States (US)

Mucin 4 antibody

GTX100775_39471_IHC_19011506_562.jpg
GTX100775_WB_19011506_487.jpg

Cat No. GTX100775

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

232 kDa. ( Note )

Positive Control

A549
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Mucin 4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HSA276359 antibody, MUC4 antibody, testis mucin antibody, pancreatic adenocarcinoma mucin antibody, "mucin 4, tracheobronchial antibody", ascites sialoglycoprotein antibody, mucin-4 antibody, tracheobronchial mucin antibody, "mucin 4, cell surface associated antibody"

Cellular Localization

Membrane; Single-pass membrane protein (Potential) , Secreted

Background

The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of this gene have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. This gene contains a region in the coding sequence which has a variable number (>100) of 48 nt tandem repeats. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100775_39471_IHC_19011506_562.jpg

GTX100775 IHC-P Image

Mucin 4 antibody detects Mucin 4 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Mucin 4 antibody (GTX100775) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100775_WB_19011506_487.jpg

GTX100775 WB Image

Sample (30 ug of whole cell lysate)
A: A549 7.5% SDS PAGE
GTX100775 diluted at 1:1000

REVIEW

Mucin 4 antibody Cat No. GTX100775

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 30-Jun-2017
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : mammary analogue secretory carcinoma
Blocking : Protein Blood Serum-free (DAKO X0909), 25°C, 0.3Hr
Fixation : 20% Formalin Neutral Buffer Solution
Permeabilization /Antigen retrieval : 10 mM sodium citrate buffer pH 6.0
Primary Antibody : 1:100, 37°C, 1Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169