GeneTex
United States (US)

Mucin 5AC antibody [SPM297]

GTX34873_20170712_IHC-P_18121410_246.jpg

Cat No. GTX34873

Host Mouse
Clonality Monoclonal
Clone Name SPM297
Isotype IgG1
Application IHC-P
Reactivity Human, Mouse, Rat, Rabbit, Cat, Chicken, Pig, Monkey, Hedgehog
APPLICATION

Application Note

IHC-P:1-2ug/ml for 30 minutes at RT.

Specificity/Sensitivity

This MAb recognizes the peptide core of gastric mucin M1 (>1,000kDa) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Immunogen

M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MUC5AC Antibody , mucin 5AC, oligomeric mucus/gel-forming Antibody
DATA IMAGES
GTX34873_20170712_IHC-P_18121410_246.jpg

GTX34873 IHC-P Image

Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Monoclonal Antibody (SPM297).

Package List Price ($)
$ 329