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Musashi 1 antibody [N3C3]

Anti-Musashi 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101540
Anti-Musashi 1 antibody [N3C3] used in IHC (Frozen sections) (IHC-Fr). GTX101540
Anti-Musashi 1 antibody [N3C3] used in Western Blot (WB). GTX101540
Anti-Musashi 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101540
Anti-Musashi 1 antibody [N3C3] used in IHC (Frozen sections) (IHC-Fr). GTX101540
Anti-Musashi 1 antibody [N3C3] used in Western Blot (WB). GTX101540
Anti-Musashi 1 antibody [N3C3] used in Western Blot (WB). GTX101540

Cat. No. GTX101540

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Musashi 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

mouse fetal brain , SH-SY-5Y

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Musashi 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950950

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

musashi RNA binding protein 1

Background

This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Musashi 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101540

GTX101540 ICC/IF Image

MSI1 antibody [N3C3] detects MSI1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MSI1 protein stained by MSI1 antibody [N3C3] (GTX101540) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Anti-Musashi 1 antibody [N3C3] used in IHC (Frozen sections) (IHC-Fr). GTX101540

GTX101540 IHC-Fr Image

Musashi 1 antibody [N3C3] detects Musashi 1 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Musashi 1 protein stained by Musashi 1 antibody [N3C3] (GTX101540) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Musashi 1 antibody [N3C3] used in Western Blot (WB). GTX101540

GTX101540 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Musashi 1 antibody [N3C3] (GTX101540) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Musashi 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101540

GTX101540 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using MSI1(GTX101540) antibody at 1:200 dilution.

Anti-Musashi 1 antibody [N3C3] used in IHC (Frozen sections) (IHC-Fr). GTX101540

GTX101540 IHC-Fr Image

MSI1 antibody [N3C3] detects MSI1 protein on adult mouse brain by immunohistochemical analysis. Sample: Frozen section of adult mouse brain. MSI1 antibody [N3C3] (GTX101540) diluted at 1:500.

Anti-Musashi 1 antibody [N3C3] used in Western Blot (WB). GTX101540

GTX101540 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Musashi 1 antibody [N3C3] (GTX101540) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Musashi 1 antibody [N3C3] used in Western Blot (WB). GTX101540

GTX101540 WB Image

The data was published in the journal Nat Commun in 2014. PMID: 24722317

REFERENCE

Application Reference

Ma L et al. Oncol Lett 2018; 16 (4) : 5441-5448 Identification of a novel splice variant of the human musashi-1 gene.
Lin Ma et al. Oncology Letters 2018; (Epub) Identification of a novel splice variant of the human musashi-1 gene.
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
Nowak JS et al. Nat Commun 2014; 5 : 3687 Lin28a regulates neuronal differentiation and controls miR-9 production.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169