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Musashi 1 antibody [N3C3]

Anti-Musashi 1 antibody [N3C3] used in IHC (Frozen sections) (IHC-Fr). GTX101540
Anti-Musashi 1 antibody [N3C3] used in Western Blot (WB). GTX101540
Anti-Musashi 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101540
Anti-Musashi 1 antibody [N3C3] used in Western Blot (WB). GTX101540
Anti-Musashi 1 antibody [N3C3] used in IHC (Frozen sections) (IHC-Fr). GTX101540
Anti-Musashi 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101540
Anti-Musashi 1 antibody [N3C3] used in Western Blot (WB). GTX101540

Cat No. GTX101540

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr, IP

Reactivity

Human, Mouse
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

SH-SY-5Y , mouse fetal brain

Predict Reactivity

Rat, Bovine, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Musashi 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

musashi RNA binding protein 1

Background

This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Musashi 1 antibody [N3C3] used in IHC (Frozen sections) (IHC-Fr). GTX101540

GTX101540 IHC-Fr Image

MSI1 antibody [N3C3] detects MSI1 protein on adult mouse brain by immunohistochemical analysis. Sample: Frozen section of adult mouse brain. MSI1 antibody [N3C3] (GTX101540) diluted at 1:500.

Anti-Musashi 1 antibody [N3C3] used in Western Blot (WB). GTX101540

GTX101540 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Musashi 1 antibody [N3C3] (GTX101540) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Musashi 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101540

GTX101540 ICC/IF Image

MSI1 antibody [N3C3] detects MSI1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MSI1 protein stained by MSI1 antibody [N3C3] (GTX101540) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-Musashi 1 antibody [N3C3] used in Western Blot (WB). GTX101540

GTX101540 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Musashi 1 antibody [N3C3] (GTX101540) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Musashi 1 antibody [N3C3] used in IHC (Frozen sections) (IHC-Fr). GTX101540

GTX101540 IHC-Fr Image

Musashi 1 antibody [N3C3] detects Musashi 1 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Musashi 1 protein stained by Musashi 1 antibody [N3C3] (GTX101540) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Musashi 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101540

GTX101540 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using MSI1(GTX101540) antibody at 1:200 dilution.

Anti-Musashi 1 antibody [N3C3] used in Western Blot (WB). GTX101540

GTX101540 WB Image

SILAC combined with RNA pull-down and Mass Spectrometry reveals putative regulators of brain-specific microRNA biogenesis(a) Schematic of the method. P19 cells were grown in “light” medium containing 12C6-arginine and 12C6-lysine or “heavy” medium containing 13C6-arginine and 13C6-lysine. Cells grown in “light” medium were subjected to retinoic acid-induced neuronal differentiation until d9. Next, RNA pull-down was performed with agarose beads covalently linked to microRNA CTLs or pre-microRNAs and incubated with premixed extracts from “light” d9 or “heavy” d0 P19 cells. After RNase treatment, the supernatants were subjected to quantitative mass spectrometry, which identifies putative microRNA biogenesis factors. (b) The graph represents the fold enrichment of proteins that bind to the miR-9-1 CTL in experiments with “heavy” d0 P19 cell extracts compared with “light” d9 P19 cell extracts. The values are presented on a log10 scale. The Lin28a protein is indicated in red. (c) Western blot analysis of miR-9-1 CTL RNA pull-down with d0 and d9 P19 cell extracts for Lin28a, MSI1 and hnRNP A1. Lanes 1 and 2 show reactions with beads alone and d0 and d9 cell extracts, respectively. Lanes 2 and 3 represent 4% (100 μg) of the loading control for d0 and d9 cell extracts, respectively. Lanes 5 and 6 represent miR-9-1 CTL RNA pull-downs with d0 and d9 cell extracts, respectively. Lanes 7 and 8 represent miR-9-1 CTL RNA pull-downs with d0 and d9 cell extracts, respectively. The results are representative of at least three independent experiments.

REFERENCE

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169