GeneTex
United States (US)

Muscle Actin antibody [HHF35]

Actin-muscle-antibody-HHF35_IHC (Formalin-fixed paraffin-embedded sections)_GTX27813-1_18121409_480.jpg

Cat No. GTX27813

Host Mouse
Clonality Monoclonal
Clone Name HHF35
Isotype IgG1
Application IHC-P, IHC-Fr
Reactivity Human, Rat, Rabbit, Hamster, Monkey
APPLICATION

Application Note

Immunohistochemistry (1:50 to 1:60): formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. Also works on frozen sections.

Specificity/Sensitivity

This antibody recognizes muscle specific alpha and gamma actin isomers but is non-reactive with beta isomers.
PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA and 0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Antigen Species

Human

Immunogen

The SDS extracted protein fraction of human myocardium.

Purification

Tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ACTA, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3

Background

This antibody stains myocardial, skeletal muscle and smooth muscle cells as well as myoepithelial cells, pericytes of small vessels. All other non-muscle cells were negative.
DATA IMAGES
Actin-muscle-antibody-HHF35_IHC (Formalin-fixed paraffin-embedded sections)_GTX27813-1_18121409_480.jpg

GTX27813 IHC-P Image

Formalin fixed paraffin embedded human leiomyoma stained with Muscle Actin antibody (cat. # GTX27813).

Package List Price ($)
$ 319