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Muscleblind-like 1 antibody

Anti-Muscleblind-like 1 antibody used in Western Blot (WB). GTX112624
Anti-Muscleblind-like 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112624

Cat No. GTX112624

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

U87-MG

Product Note

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Muscleblind-like 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169979

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

muscleblind like splicing regulator 1 , EXP , MBNL

Cellular Localization

Nucleus

Background

Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.

Database

Research Area

DATA IMAGES

Anti-Muscleblind-like 1 antibody used in Western Blot (WB). GTX112624

GTX112624 WB Image

Sample (30 μg of whole cell lysate)
A: U87-MG
10% SDS PAGE
GTX112624 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Muscleblind-like 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112624

GTX112624 ICC/IF Image

Muscleblind-like 1 antibody detects Muscleblind-like 1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Muscleblind-like 1 stained by Muscleblind-like 1 antibody (GTX112624) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169