APPLICATION
Application Note
We recommend using the capture antibody at a concentration of 5μg/mL and the detection antibody at a concentration of 5μg/mL. Optimal dilutions should be determined experimentally by the researcher.
PROPERTIES
Form
Liquid
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Myd88, Innate Immune Signal Transduction Adaptor , Myd88D , Myd88
Background
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.
Research Area
DATA IMAGES
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GTX500015 ELISA Image
ELISA detection of MyD88 using GTX112986 for capture at a concentration of 5 μg/mL and GTX89605 for detection at a concentration of 5 μg/mL. The HRP-conjugated anti-goat IgG antibody (GTX26741) was diluted at 1:20,000-1:200,000 and used to detect the primary antibody.
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REFERENCE
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REVIEW
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