GeneTex
United States (US)

MyD88 antibody

MYD88_ELISA-PAIR_18121411_180.jpg
GTX112986_40177_20151111_IFA_18121410_735.jpg
GTX112986_40177_20171006_WB_B_18121410_397.jpg

Cat No. GTX112986

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA, sELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
ELISA 1:1000-1:10000
sELISA Assay dependent

Note :

sELISA
Capture: GTX112986 , Detection: GTX89605

Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

MYD88-transfected 293T

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MYD88. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Myd88, Innate Immune Signal Transduction Adaptor,Myd88D,Myd88

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES
MYD88_ELISA-PAIR_18121411_180.jpg

GTX112986 ELISA Image

ELISA detection of MyD88 using GTX112986 for capture at a concentration of 5 µg/mL and GTX89605 for detection at a concentration of 5 µg/mL.

GTX112986_40177_20151111_IFA_18121410_735.jpg

GTX112986 ICC/IF Image

MYD88 antibody detects MYD88 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min.
Green: MYD88 protein stained by MYD88 antibody (GTX112986) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX112986_40177_20171006_WB_B_18121410_397.jpg

GTX112986 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with MyD88 antibody (GTX112986) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169