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MyD88 antibody

Anti-MyD88 antibody used in ELISA (ELISA). GTX112986
Anti-MyD88 antibody used in Western Blot (WB). GTX112986
Anti-MyD88 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112986

Cat. No. GTX112986

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA, Sandwich ELISA

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MyD88 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX112986 , Detection: GTX89605
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

MYD88-transfected 293T

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MYD88. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617657

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MYD88 innate immune signal transduction adaptor , MYD88D

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-MyD88 antibody used in ELISA (ELISA). GTX112986

GTX112986 ELISA Image

ELISA detection of MyD88 using GTX112986 for capture at a concentration of 5 μg/mL and GTX89605 for detection at a concentration of 5 μg/mL.

Anti-MyD88 antibody used in Western Blot (WB). GTX112986

GTX112986 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MyD88 antibody (GTX112986) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MyD88 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112986

GTX112986 ICC/IF Image

MYD88 antibody detects MYD88 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min.
Green: MYD88 protein stained by MYD88 antibody (GTX112986) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Lai YH et al. Oncol Rep 2019; 41 (2) : 999-1006 Mitochondrial?DNA?associated TLR9 signalling is a potential serological biomarker for non?small cell lung cancer.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169