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MyD88 antibody

Orthogonal
Anti-MyD88 antibody used in Western Blot (WB). GTX112987
Anti-MyD88 antibody used in IHC (Paraffin sections) (IHC-P). GTX112987
Anti-MyD88 antibody used in IHC (Frozen sections) (IHC-Fr). GTX112987

Cat. No. GTX112987

Reference 9

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MyD88 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

MyD88-transfected 293T

Predict Reactivity

Rat, Sheep, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MYD88. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10720430

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MYD88 innate immune signal transduction adaptor , MYD88D

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-MyD88 antibody used in Western Blot (WB). GTX112987

GTX112987 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MyD88 antibody (GTX112987) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MyD88 antibody used in IHC (Paraffin sections) (IHC-P). GTX112987

GTX112987 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung papillory cancer, using MYD88(GTX112987) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MyD88 antibody used in IHC (Frozen sections) (IHC-Fr). GTX112987

GTX112987 IHC-Fr Image

Orthogonal

The data was published in the journal Eur J Neurosci in 2014.PMID: 24754835

REFERENCE

Application Reference

W Liang et al. Probiotics Antimicrob Proteins 2023; Lactiplantibacillus plantarum ELF051 Alleviates Antibiotic-Associated Diarrhea by Regulating Intestinal Inflammation and Gut Microbiota
Application : WB
Liu G et al. Evid Based Complement Alternat Med 2022; 2022 : 5886698 Uncovering the Mechanism of the Xingnaojing Injection against Ischemic Stroke Using a Combined Network Pharmacology Approach and Gut Microbiota Analysis.
Kang-Hsi Wu et al. Int J Mol Sci 2022; 23 (10) Immunomodulation via MyD88-NFκB Signaling Pathway from Human Umbilical Cord-Derived Mesenchymal Stem Cells in Acute Lung Injury.
Yen IC et al. Biomed Pharmacother 2021; 138 : 111504 4-Acetylantroquinonol B ameliorates nonalcoholic steatohepatitis by suppression of ER stress and NLRP3 inflammasome activation.
Application : WB
Reactivity : Mouse
Karunarathne WAHM et al. Environ Pollut 2021; 278 : 116829 Bisphenol A: A potential Toll-like receptor 4/myeloid differentiation factor 2 complex agonist.
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REVIEW

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Application Reference
W Liang et al. Probiotics Antimicrob Proteins 2023; Lactiplantibacillus plantarum ELF051 Alleviates Antibiotic-Associated Diarrhea by Regulating Intestinal Inflammation and Gut Microbiota
Application : WB
Liu G et al. Evid Based Complement Alternat Med 2022; 2022:5886698 Uncovering the Mechanism of the Xingnaojing Injection against Ischemic Stroke Using a Combined Network Pharmacology Approach and Gut Microbiota Analysis.
Kang-Hsi Wu et al. Int J Mol Sci 2022; 23 (10) Immunomodulation via MyD88-NFκB Signaling Pathway from Human Umbilical Cord-Derived Mesenchymal Stem Cells in Acute Lung Injury.
Yen IC et al. Biomed Pharmacother 2021; 138:111504 4-Acetylantroquinonol B ameliorates nonalcoholic steatohepatitis by suppression of ER stress and NLRP3 inflammasome activation.
Application : WB
Reactivity : Mouse
Karunarathne WAHM et al. Environ Pollut 2021; 278:116829 Bisphenol A: A potential Toll-like receptor 4/myeloid differentiation factor 2 complex agonist.
Wu KH et al. Lupus 2020; 29 (2):165-175 Toll-like receptor signalling associated with immunomodulation of umbilical cord-derived mesenchymal stem cells in mice with systemic lupus erythematosus.
Application : WB
Reactivity : Mouse
Lee SC et al. PLoS One 2015; 10 (3):e0120700 Protective effect of leaf essential oil from Cinnamomum osmophloeum Kanehira on endotoxin-induced intestinal injury in mice associated with suppressed local expression of molecules in the signaling pathways of TLR4 and NLRP3.
Application : WB
Reactivity : Mouse
Liu YH et al. Mar Drugs 2015; 13 (2):1026-36 Omega-3 fatty acid intervention suppresses lipopolysaccharide-induced inflammation and weight loss in mice.
Application : WB
Reactivity : Mouse
Dvoriantchikova G et al. Eur J Neurosci 2014; the tir-domain-containing adapter inducing *-β-dependent signaling cascade plays a crucial role in ischemia-reperfusion-induced retinal injury, whereas the contribution of the myeloid differentiation primary response 88-dependent signaling cascade is not as pivotal.
Application : IHC-Fr
Reactivity : Mouse
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169
Currently in production